雄黄遗传毒性的研究

目的评价雄黄的急性毒性和遗传毒性,为临床提供毒理学依据。方法急性毒性:设3.50、2.98、2.53、2.15、1.83、1.55g/kg剂量组灌胃,对照组给予等体积的0.5%羧甲基纤维素钠溶液(Sodium Carboxymethyl Cellulose,CMC)。Ames试验:设雄黄浸出液原液、1:1、3:1、7:1和15:1稀释作为中、低、次低和最低剂量组,各剂量组均加不同浓度的雄黄浸出液0.5ml,观察加或不加S9混合物对TA97、TA98、TA100、TA102、TA1535菌株的影响。小鼠骨髓嗜多染红细胞微核试验:设1、0.5、0.25g/kg三个剂量组,2次灌胃后,24h制片,镜检。CHL细胞染色体畸形试验:设雄黄浸出液1:15、1:31、1:63稀释液三个剂量,加药后将细胞放入CO2培养箱中37℃培养24-48小时。终止培养前4小时,每只细胞培养皿中加入秋水仙素溶液0.1ml,收获细胞,制染色体标本、镜检。结果急性毒性试验表明:本次实验雄黄的LD50为2.069g/kg。Ames试验:为阴性结果,雄黄有抑制细菌生长的作用。雄黄对小鼠骨髓嗜多染红细胞有致突变作用和对CHL细胞染色体有致畸变作用。结论本次试验中该批次的雄黄有一定的遗传毒性。

Study on Genetic Toxicity of Realgar

Objective To provide toxicity data of realgar for clinic application.Methods Acute toxicity:groups of mice for six doses(3.50、2.98、2.53、2.15、1.83、1.55g/kg) were administrated by gavage,negative was 0.5%CMC.Ames test:groups for five doses(leaching solution,1:1、3:1、7:1and 15:1 dilution,(0.5ml/plate) were divided to observe the effects of realgar on strains of TA 97,TA98,TA 100,TA 102,TA1535 with or without S9 mixture added.Micronucleus test of bone marrow PCE cell in mice:Three groups for 1、0.5、0.25 g/kg were divided.After the animals received realgar by gavage two times,they were killed.Then the slides were made and observed.In vitro mammalian cells chromosome aberration test:three groups for leaching solution1:15and1:31and1:63.Gave the realgar and cultured 24 hours or 48 hours,then collected these cells before the four hours gave the colchicine tablets to make the cell smear specimens and stained them after fixation.The frequency of chromosome aberration was calculated in 200 metaphase cells.Results Realgar was a substance with toxicity according to the acute toxicity.The LD50 was 2.069g/kg.Ames test:the result of realgar was negative.realgar had mutagenesis on bone marrow PCE cells in mice,and mutagenesis on chromosome.Conclusion The toxicity of this realgar has genetic toxicity.