成年小鼠脑室下区神经干细胞培养与鉴定体系的建立

目的 建立系统的成年小鼠脑室下区神经干细胞分离、培养及鉴定体系.方法 用无血清方法分离培养成年小鼠脑室下区来源的神经干细胞;用克隆培养、BrdU整合的方法检验培养细胞的干细胞特性;用免疫荧光细胞化学方法检测Brdu、神经干细胞标记物nestin和SOX2,分化的细胞标记物Tuj1、GFAP、NG2.用Western boltting和RT-PCR方法进一步检测神经干细胞标记物nestin和SOX2的表达.结果 从成年小鼠脑室下区分离培养出具有自我更新、增殖的神经球,构成神经球的细胞nestin和SOX2呈阳性,它们分化后产生Tujl阳性的神经元、GFAP阳性的星型胶质细胞、NG2阳性的少突胶质细胞.结论 建立了简单、稳定的培养成年小鼠脑室下区神经干细胞方法.

Culture and identification of neural stem cells derived from the subventricular zone of adult mice

Objective To establish a method for culturing and identifying neural stem cells (NSCs) derived from the subventricular zone (SVZ) in adult mice.Methods NSCs were isolated from the SVZ of adult mouse brain and cultured in serum-free medium.Cell cloning and BrdU incorporation were performed to identify the self-renewal and proliferative capacity of the NSCs.Fluorescence immunocytochemistry was used to examine the expressions of the NSC markers nestin and SOX2,neuronal marker Tuj1,astrocyte marker GFAP and oli...