三阴乳腺癌中C—myc和Skp2的表达及相关性研究

目的检测三阴乳腺癌（TNBC）组织中C．myc和Skp2的表达情况及其意义。方法选取2008年1月一2011年12月手术切除的TNBC标本56例，采用免疫组织化学链霉素抗生物素蛋白．过氧化物酶连结法检测它们和30例癌旁正常乳腺组织中C．myc和sk02的表达情况。结果TNBC组织中C．myc和Skp2表达定位于细胞核，其表达率分别为57．14％（32／56）和58．93％（33／56），明显高于癌旁正常组织（P〈0．05）；56例TNBC组织中，C-myc和skp2蛋白在组织学分级Ⅲ级表达率高于I＋Ⅱ级（P〈0．05）；C．myc和skp2的病理分期Ⅲ期的表达率高于I-Ⅱ期（P〈O．05）；表达率高低与腋窝有无淋巴结转移有关（P〈0．05），而与病理组织类型无关（P〉O．05）；相关性分析显示，C．myc和Skp2二者之间呈正相关（P〈O．05）。结论C．myc和Skp2在TNBC中呈明显高表达，与TNBC的发生、发展和转移密切相关。

Expression and Significance of C-myc and Skp2 in Triple-Negative Breast Cancer

Objective To investigate the expression of C-myc and Skp2 in triple-negative breast cancer （TNBC）, and to discuss its clinical pathological significance. Methods From January 2008 to December 2011, by using streptavidin-perosidase immunohistochemistry method, the expression of C-myc and Skp2 in 56 TNBC cases and 30 breast tissue adjacent to cancer eases were examined. Results The expression of C-myc and Skp2 in human breast cancer was located within the nucleus. The positive expression rates of C-mye and Skp2 were 57.14% （32/56） and 58.93% （33/56） respectively in TNBC which were higher than that in the adjacent normal tissue （P 〈 0.05）. In the 56 cases of TNBC, the positive expression of C-mye and Skp2 in histological grade G3 was significantly higher than that in G1 and G2 （P 〈 0.05）. In addition, the positive expression of C-mye and Skp2 in stage III - IV tumors was also higher than that in stage I - II tumors （P 〈 0.05）. Furthermore, the expression of C-myc and Skp2 were associated with axillary lymph node metastasis （P 〈 0.05）, and were not related with histopathologieal type （P 〉 0.05）. Correlation analysis showed a positive correlation between C-myc and Skp2 （P 〈 0.05）. Conclusion The high expression of C-myc and Skp2 are found in TNBC, which are closely related with the occurrence, development and metastasis of TNBC.