Effect of the hapten linking arm on cross-reactivity of sulfonamide-specific antibodies in ELISA |
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Authors: | Gordon A. Grant Peter Sporns |
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Affiliation: | a Department of Agricultural, Food and Nutritional Science, University of Alberta, Alberta, Edmonton, Canada |
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Abstract: | Competitive enzyme-linked immunosorbent assay (ELISA) was used to explore the influence of the linking arm (azo or succinyl) of sulfonamide-protein immunogens on polyclonal antibody (pAb) cross-reactivity to sulfonamide N1-analogues, N4-derivatives, or N4-protein conjugates. In addition to the sulfonamide's overall size and N1-substituent structure, the dominant sulfonamide epitope of the immunogen was defined by the linking arm. Antibodies made from immunizations with azo and succinyl immunogens possessed 12- and 16-fold greater affinity for their N4-derivatives, respectively, compared to the underivatized sulfonamide form. |
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Keywords: | Polyclonal antibody hapten design linking arm effect cross-reactivity sulfonamide |
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