麝香醒脑宁对脑缺血再灌注损伤的保护作用及机制

目的:探讨麝香醒脑宁(Shexiang Xingnaonin,SXN)对脑缺血再灌注损伤的保护作用及其机制。方法:采用大鼠线栓法制备脑缺血再灌注模型,观察SXN对神经功能评分、脑组织病理形态学变化的影响,电镜观察脑组织超微结构,免疫组化检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达,血栓形成仪测定体外血栓形成及比值法测定血小板聚集;体外法观察SXN对血凝块的溶解作用。结果:与模型组比较,SXN(0.04,0.08,0.16 g.kg^-1)可降低脑缺血再灌注8 h和22 h的神经功能评分,改善脑组织病理形态学和超微结构,抑制脑组织TNF-α的表达;SXN(0.08,0.16 g.kg^-1)对体外血栓形成和血小板聚集有一定的抑制作用;SXN(0.16,0.32 g.L^-1)在体外对血凝块有较好的溶解作用。结论:SXN对脑缺血再灌注损伤具有一定的保护作用,其机制可能与抑制TNF-α的表达、抑制血栓形成等有关。

Protective effects of Shexiang Xingnaonin on focal cerebral ischemia/reperfusion injury and mechanism

OBJECTIVE: To study the protective effects of Shexiang Xingnaonin (SXN) on focal cerebral ischemia/reperfusion injury and its mechanism. METHOD: Middle cerebral artery occlusion (MCAO) was used to make focal cerebral ischemia/reperfusion model by in travascular nylon filament occlusion. The protective effects of SXN at different doses were evaluated by investigating neurological function score, pathomorphology of brain, the ultrastructure of neuron, expression of tumor necrosis factor-alpha, thrombogenesis in vitro, platelet aggregation and lysing effect of blood clot in vitro. RESULT: Compared with model group, SXN (0.08, 0.16 g x kg(-1)) could decrease the neurological score, improve pathomorphology and neuron ultrastructure of brain, inhibit the expression of TNF-alpha, decrease the length, wet weight and dry weight of thromb and inhibit platelet aggregation. And SXN (0.16, 0.32 g x L(-1)) could dissolve blood clot in vitro. CONCLUSION: SXN has protective effects on focal cerebral ischemia/reperfusion injury. The role of inhibit the expression of TNF-alpha, inhibit thrombogenesis and platelet aggregation might contribute to its neuroprotective effects.