HPLC法同时测定消炎片中绿原酸、秦皮乙素和咖啡酸的含量

目的:建立HPLC法同时测定消炎片中绿原酸、秦皮乙素和咖啡酸的含量.方法:采用Agilent Eclipse XDB-C18 (250mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-0.04％磷酸溶液为流动相,梯度洗脱；检测波长为328 nm；流速1.0ml·min -1；进样量20μl；柱温35℃.结果:绿原酸、秦皮乙素和咖啡酸的线性范围分别为1.616 ～40.400μg·ml-1(r=0.999 9,n=7)、5.164～129.100μg ·ml-1(r=1.000 0,n=7)和1.344～ 26.880 μg·ml-1(r=1.000 0,n=7)；提取回收率分别为99.08％(RSD=0.6％,n=6)、98.81％(RSD =0.4％,n=6)和98.92％(RSD=0.9％,n=6).结论:本法操作简便、快速、结果准确.

Simultaneous Determination of Chlorogenic Acid, Esculetin and Caffeic Acid In Xiaoyan Tablets By HPLC

Objective： To establish a RP - HPLC method for the simultaneous determination of chlorogenic acid,esculetin and caffeic acid in Xiaoyan tablets. Method： An Agilent Eclipse XDBTC18 （250mm×4.6mm,5μm） column was used. The mobile phase was methanol - acetonitrile-0. 04% H3 PO4 with gradient elution and the detection wavelength was 328 nm, the flow rate was 1.0 ml. min-1, the injection volume was 20 μl and the column temperature was 35℃. Result： The linear response of chlorogenic acid range was from 1.616-40. 400μg·ml-1 （ r = 0. 999 9, n = 7 ）, and that for esculetin and caffeic acid was from 5. 164-129. 100 Ixg＂ ml - 1 （ r = 1. 000 0, n =7） and 1. 344-26. 880μg·ml-1 （ r = 1. 000 0, n = 7 ）, respectively. The average recovery was 99. 08% （ RSD = 0.6% , n = 6）, 98. 81% （ RSD = 0.4%, n = 6 ） and 98.92% （ RSD = 0. 9%, n = 6 ） accordingly. Conclusion： The method is convenient, quick and accurate.