| HPLC法同时测定野菊花中绿原酸和蒙花苷的含量 |
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| 引用本文： | 陈立柱,刘辉,潘鹏飞.HPLC法同时测定野菊花中绿原酸和蒙花苷的含量[J].中国药师,2011,14(12):1753-1755. |
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| 作者姓名： | 陈立柱 刘辉 潘鹏飞 |
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| 作者单位： | 1. 鸡西市食品药品检验检测中心 黑龙江鸡西158100
2. 鸡西市传染病医院 |
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| 摘 要： | 目的:建立HPLC法同时测定野菊花中绿原酸和蒙花苷的含量。方法:色谱柱:Agilent Eclipse XDB-C18（250 mm×4.6 mm,5μm）;流动相:甲醇-乙腈-0.05%磷酸溶液,梯度洗脱;检测波长:326 nm;流速:1.0 ml·min-1;进样量:10μl;柱温:35℃。结果:绿原酸和蒙花苷的线性范围分别为3.476×10-2～0.695 2μg（r=1.000 0,n=7）和8.957×10-2～1.792μg（r=1.000 0,n=7）;提取回收率分别为98.79%（RSD=1.0%,n=6）和99.39%（RSD=0.5%,n=6）。结论:本法操作简便、快速、结果准确,可用于野菊花的质量控制。
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| 关 键 词： | 野菊花 含量测定 绿原酸 蒙花苷 高效液相色谱法 |
Simultaneous Determination of Chlorogenic Acid and Buddleoside in Flos Chrysanthemi Indici by HPLC |
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| Chen Lizhu
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Liu Hui
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Pan Pengfei.Simultaneous Determination of Chlorogenic Acid and Buddleoside in Flos Chrysanthemi Indici by HPLC[J].China Pharmacist,2011,14(12):1753-1755. |
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| Authors: | Chen Lizhu Liu Hui Pan Pengfei |
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| Institution: | (I. Jixi Food and Drug Testing Center, Heilongjiang Jixi 158100, China;2. Jixi Infectious Disease Hospital) |
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| Abstract: | Objective： To establish a RP-HPLC method for the simultaneous determination of chlorogenic acid and buddleoside in Flos Chrysanthemi lndici. Method： An Agilent Eclipse XDB-C18 （250mm×4.6mm,5μm） column was used. The mobile phase was methanol-acetonitrile-0. 05% H3PO4 with gradient elution and the detection wavelength was 326 nm. The flow rate was 1.0 ml.min-I, the injection volume was 10μl and the column temperature was 35℃. Result： The linear response of chlorogenic acid ranged from 3.476 × 10-2-0. 695 2 μg （r = 1. 000 0,n =7） ,and that for buddleoside was 8. 957 × 10-2-1. 792 μg （r = 1. 000 0,n =7）. The average recovery of chlorogenic acid and buddleoside was 98. 79% （RSD = 1.0% ,n =6） and 99. 39% （RSD =0. 5% ,n =6） ,respectively. Conclusion： The method is convenient,rapid and accurate, and can be used for the quality control of Flos Chrysanthemi lndici. |
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| Keywords: | Flos chrysanthemi indici Determination Chlorogenic acid Buddleoside HPLC |
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