T细胞亚群在儿童喘息性疾病中表达的研究

目的 探讨喘息性疾病患儿外周血T淋巴细胞亚群的表达及意义。 方法 应用流式细胞仪检测哮喘急性发作组、喘息性肺炎组及正常对照组儿童外周血T细胞亚群表达水平。结果 哮喘急性发作组和喘息性肺炎组CD3⁺淋巴细胞百分比均较正常对照组明显降低(P<0.01),且二者降低水平一致(P>0.05)。哮喘急性发作组CD4⁺淋巴细胞百分比较正常对照组和喘息性肺炎组均明显增高(P<0.05),且喘息性肺炎组CD4⁺淋巴细胞百分比与对照组比较差异也有统计学意义(P<0.05)。哮喘急性发作组及喘息性肺炎组CD8⁺淋巴细胞百分比与正常对照组比较明显降低(P<0.05),而哮喘急性发作组及喘息性肺炎组之间差异无统计学意义(P>0.05)。哮喘急性发作组和普通喘息组CD3^-CD19⁺和CD19⁺CD23⁺较正常对照组明显升高,差异有统计学意义(P<0.05),但这些指标在哮喘急性发作组和普通喘息组之间差异均无统计学意义(P>0.05)。 结论细胞免疫功能紊乱参与儿童喘息性疾病的发病。

Effect of T-lymphocyte subsets in children with asthmatic disease

Objective To explore the expression of T-lymphocyte subsets in peripheral blood of children with asthmatic disease. Method Flow cytometry (FCM) was applied to measure the expression of T-lymphocytes in children within acute asthma group,asthmatic pneumonia group and healthy control group. Results Percentages of CD3⁺ lymphocytes of acute asthma group and asthmatic pneumonia group were significantly lower than that of healthy control group (P<0.01),with no statistical variance found between acute asthma group and asthmatic pneumonia group (P>0.05).Compared to healthy control group,percentage of CD4⁺ lymphocytes in acute asthma group and asthmatic pneumonia group were significantly higher (P<0.05),with significant differences between asthmatic pneumonia group and healthy control group.Compared with healthy control group,percentages of CD8⁺ lymphocytes was significantly lower than that in acute asthma group and asthmatic pneumonia group (P<0.05),with no significant difference between acute asthma group and asthmatic pneumonia group (P>0.05).CD3^-CD19⁺and CD19⁺CD23⁺ lymphocytes of acute asthma group and asthmatic pneumonia group were markedly increased than those of healthy control group (P<0.05),but variances of these two subsets in exacerbation asthma group and asthmatic pneumonia group were not statistically significance (P>0.05).Conclusion Cellular immunity disorder was involved in the pathogenesis in children with asthma and asthmatic pneumonia.