结核分枝杆菌融合抗原38F和64F诊断活动性肺结核的价值

目的 探讨结核分枝杆菌融合抗原38F和64F对活动性肺结核患者血清中IgG、IgM和IgA抗体检测的诊断价值。 方法 利用结核分枝杆菌抗原优势肽段融合抗原38F和64F，通过ELISA法对223例活动性肺结核患者进行结核分枝杆菌特异性IgG、IgM和IgA抗体水平检测。223例活动性肺结核患者分为三组，第一组为涂片和培养共同阳性组（86例），第二组为涂片阴性且培养阳性组（51例），第三组为涂片和培养共同阴性组（86例）。 结果223例活动性肺结核病患者，第一组IgG、IgM和IgA的联合检出率为79.07%（68/86）；第二组IgG、IgM和IgA的联合检出率为62.75%（32/51）；第三组IgG、IgM和IgA的联合检出率为69.77%（60/86）。全部样本血清学方法的检出率为71.75%（160/223）。结论 结核分枝杆菌融合抗原38F和64F对于活动性肺结核具有较高的辅助诊断价值，并且IgG、IgM和IgA抗体联合检测可以提高检出率。

The value of Mycobacterium tuberculosis fusion antigen 38F and 64F in the diagnosis of active tuberculosis

Objective To explore the diagnostic value of Mycobacterium tuberculosis fusion antigen 38F and 64F in detecting IgG, IgM and IgA antibody in serum of patients with active pulmonary tuberculosis. Methods By using enzyme-linked immuno sorbent assay （ELISA） method, IgG, IgM and IgA antibody levels in serum of 223 pa- tients with active pulmonary tuberculosis were detected with the Mycobacterium tuberculosis seven immune-dominat peptide fusion antigen 38F and 64F. Patients with active pulmonary tuberculosis were divided into three groups. The first group was co-positive cases of smear and culture examination （n=86）. The second group was smear-negative and culture-positive （n= 51）. The third group was co-negative cases of smear and culture examination （n=86）. Results The joint detection rate of IgG, IgM and IgA was 79.07% （68/86） for the first group, 62.75% （32/51） for the second group and 69.77% （60/86） for the third group. The detection rate of all samples by serological method was 71.75% （160/223）. Conclusion Mycobacterium tuberculosis fusion antigen 38F and 64F have high as- sistant diagnostic values to active pulmonary tuberculosis, and the detection rate is increased significantly through joint detection of IgG, IgM and IgA antibody.