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锌指蛋白ZFX在儿童B系急性淋巴细胞性白血病中的表达及意义
引用本文:王卓,梁欢欢,李本尚,黄小航,张婧,王翔,丁丽霞,江华. 锌指蛋白ZFX在儿童B系急性淋巴细胞性白血病中的表达及意义[J]. 中国当代儿科杂志, 2013, 15(7): 509-513. DOI: 10.7499/j.issn.1008-8830.2013.07.002
作者姓名:王卓  梁欢欢  李本尚  黄小航  张婧  王翔  丁丽霞  江华
作者单位:王卓,梁欢欢,李本尚,黄小航,张婧,王翔,丁丽霞,江华
基金项目:国家自然科学基金,上海高校选拔培养优秀青年教师科研专项基金
摘    要:目的:研究儿童B系急性淋巴细胞性白血病(B-ALL)患者骨髓单个核细胞中X染色体耦联锌指蛋白(ZFX)的表达及其与白血病预后的关系。方法:通过Western blot方法检测白血病细胞株(REH、HL-60、NB4、K562)中ZFX蛋白的表达;利用PCR方法扩增获得全长ZFX,并测序验证;通过实时定量PCR检测82例初发B-ALL儿童骨髓样本,24例B-ALL缓解期样本,以及64例骨折或先天性心脏病手术患儿样本(对照组)ZFX的表达;并根据治疗3年内有无骨髓或中枢神经系统复发分为预后好组和预后差组,比较两组患儿初发时骨髓中ZFX mRNA的表达水平。结果:ZFX蛋白在白血病细胞株REH、HL-60、NB4和K562中均有表达。初发ALL组骨髓中ZFX mRNA表达高于对照组(P<0.01);ALL缓解组ZFX mRNA表达明显低于ALL初发组(P<0.01);预后差的ALL患儿初发骨髓中ZFX mRNA的表达高于预后好的患儿(P<0.05)。结论ZFX在儿童B-ALL中高表达,且预后差组表达相对较高,提示它可能在B-ALL预后判断中有重要意义。

关 键 词:X染色体耦联锌指蛋白  淋巴细胞性白血病  儿童

Expression of zinc finger protein X-linked in childhood B lineage acute lymphoblastic leukemia
WANG Zhuo,LIANG Huan-Huan,LI Ben-Shang,HUANG Xiao-Hang,ZHANG Jing,WANG Xiang,DING Li-Xi,JIANG Hua. Expression of zinc finger protein X-linked in childhood B lineage acute lymphoblastic leukemia[J]. Chinese journal of contemporary pediatrics, 2013, 15(7): 509-513. DOI: 10.7499/j.issn.1008-8830.2013.07.002
Authors:WANG Zhuo  LIANG Huan-Huan  LI Ben-Shang  HUANG Xiao-Hang  ZHANG Jing  WANG Xiang  DING Li-Xi  JIANG Hua
Affiliation:WANG Zhuo, LIANG Huan-Huan, LI Ben-Shang, HUANG Xiao-Hang, ZHANG Jing, WANG Xiang, DING Li-Xia, JIANG Hua
Abstract:Objective To study the expression of zinc finger protein X-linked(ZFX) in bone marrow mononuclear cells(BMMCs) of children with B lineage acute lymphoblastic leukemia(B-ALL)and its relationship with prognosis.Methods The expression of ZFX in human leukemia cell lines(REH,HL-60,NB4 and K562) was measured by Western blot.ZFX gene was cloned by PCR from one patient and DNA sequencing technology was used to confirm it.Real-time PCR was used for detecting ZFX mRNA expression in the BMMCs of 82 children with newly-diagnosed B-ALL,24 children with complete remission(CR) after induction therapy and 64 control children(fracture or congenital heart disease patients).According to the presence of bone marrow or central nervous system relapse during a follow-up of 3 years,the patients were identified as having a good or poor prognosis.Their ZFX mRNA levels in BMMCs at diagnosis were compared.Results ZFX protein was expressed in human leukemia cell lines REH,HL-60,NB4 and K562.ZFX mRNA expression was significantly higher in the newly-diagnosed ALL group than in the control group(P<0.01).ZFX mRNA expression in the ALL CR group was significantly reduced compared with the newly-diagnosed ALL group(P<0.01).Children with a poor prognosis had significantly higher ZFX mRNA levels at diagnosis than those with a good prognosis(P<0.05).Conclusions ZFX is over-expressed in children with B-ALL and its levels are higher in those with a poor prognosis than those with a good prognosis,which suggests that ZFX is important in the prognosis evaluation of B-ALL.
Keywords:Zinc finger protein X-linked  Lymphoblastic leukemia  Child
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