血管内皮生长因子165转染骨髓间充质干细胞绿色荧光蛋白的表达

背景：血管内皮生长因子是一种有效的血管形成和通透性诱导因子，其中在体内以血管内皮生长因子165和血管内皮生长因子121表达为主，具有强烈的促血管新生作用。目的：观察以血管内皮生长因子165基因转染骨髓间充质干细胞，继而分化为血管内皮细胞的可行性。方法：分离提取50gSD大鼠骨髓间充质干细胞，采用流式细胞仪鉴定，将携有血管内皮生长因子165基因的质粒pGLV．EFla，采用慢病毒转染骨髓间充质干细胞，转染后于荧光显微镜下观察绿色荧光蛋白表达情况。结果与结论：转染后12h可见细胞内有绿色荧光蛋白表达，48h后表达增多，72h后达到高峰，其后部分细胞荧光开始减退。结果证明血管内皮生长因子165基因转染骨髓间充质干细胞后，骨髓间充质干细胞内有绿色荧光蛋白表达，提示细胞转染成功，骨髓间充质干细胞定向分化为血管内皮细胞具有可行性。

Green fluorescent protein expression in vascular endothelial growth factor 165 gene transfected bone marrow mesenchvmal stem cells

BACKGROUND： Vascular endothelial growth factor is a potent angiogenesis and permeability inducible factor. Vascular endothelial growth factor 165 and vascular endothelial growth factor 121 are mainly expressed in vivo, with a strong role of angiogenesis. OBJECTIVE： To observe the feasibility of vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells to differentiate into vascular endothelial i;ells. METHODS： Bone marrow derived mesenchymal stem cells were isolated and collected from 50 g Sprague-Dawley rats,and identified by flow cytometry. The plasmid pGLV-EFla carrying a vascular endothelia growth factor 165 gene was transfected to the mesenchymal stem cells using lentiviral. Expression of green fluorescent protein was observed under a fluorescence microscope. RESULTS AND CONCLUSION： After 12 hours of transfection, expression of green fluorescent protein was observed, increased at 48 hours, peaked at 72 hours and gradually declined thereafter. Results prove that vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells have the expression of green fluorescent protein, indicating successful transfection. It is feasible to induce bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells.