| Nogo(N-18)在大鼠海马及大脑皮质中分布和发育的免疫组织化学研究 |
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| 引用本文： | 鄂玲玲,周长满,杨磊. Nogo(N-18)在大鼠海马及大脑皮质中分布和发育的免疫组织化学研究[J]. 解剖学报, 2003, 34(3): 298-301 |
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| 作者姓名： | 鄂玲玲 周长满 杨磊 |
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| 作者单位： | 北京军医学院解剖学教研室,北京,100071;北京军医学院解剖学教研室,北京,100071;北京大学基础医学院解剖学与组织学胚胎学系,北京,100083;北京大学基础医学院解剖学与组织学胚胎学系,北京,100083 |
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| 基金项目： | 全军医学科研基金杰出人才项目 ( 0 1J0 18)，北京大学“985”行动计划重大项目 ( 70 7 68914 3 ) |
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| 摘 要： | 目的 观察Nogo(N-18)在成年大鼠、乳鼠和胎鼠海马及大脑皮质中的分布规律，探讨其存在的意义。方法 采用抗Nogo(N-18)多克隆抗体免疫组织化学链霉卵白素-过氧化物酶SP(streptavidin peroxidase，SP)法。结果 成年大鼠海马以及大脑皮质神经元胞核Nogo(N-18)免疫反应呈强阳性，胞浆和突起的反应较弱，而乳鼠和胎鼠海马及大脑皮质神经元Nogo(N-18)免疫反应阳性产物只存在于突起和胞浆中。结论 Nogo(N-18)免疫反应阳性神经元广泛分布于成年大鼠、乳鼠和胎鼠海马及大脑皮质，其生物学意义有待探讨。
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| 关 键 词： | Nogo 免疫组织化学 海马 大脑皮质 大鼠 |
DISTRIBUTION AND DEVELOPMENT OF Nogo (N-18) IMMUNOREACTIVITY IN THE HIPPOCAMPUS AND CEREBRAL CORTEX OF RAT |
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| E Ling ling ,ZHOU Chang man ,,YANG Lei. DISTRIBUTION AND DEVELOPMENT OF Nogo (N-18) IMMUNOREACTIVITY IN THE HIPPOCAMPUS AND CEREBRAL CORTEX OF RAT[J]. Acta Anatomica Sinica, 2003, 34(3): 298-301 |
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| Authors: | E Ling ling ZHOU Chang man YANG Lei |
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| Affiliation: | E Ling ling 1,ZHOU Chang man 1,2*,YANG Lei 2 |
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| Abstract: | Objective The distributive feature of Nogo(N 18) in the hippocampus and cerebral cortex of the rats was observed in order to reveal the significance of Nogo(N 18) expression in these regions. Methods Nogo(N 18)antiserum immunohistochemical SP (streptavidin peroxidase,SP) staining technique was used in the present study. Results The positive Nogo(N 18) immunoreactivity was very strong in neuronal nuclei of hippocampus and cerebral cortex of adult rat, but weaker in cell bodies and processes. In contrast, in fetal and neonatal rat, hippocampus and cerebral cortex neuronal bodies and processes were positive for the Nogo(N 18), but neuronal nuclei was negative.Conclusion Nogo(N 18) containing neurons are widely distributed in the hippocampus and cerebral cortex of adult and fetal and neonatal rats.;[ |
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| Keywords: | Nogo Immunohistochemistry Hippocampus Cerebral cortex Rat |
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