尼美舒利对胆管癌细胞增殖及PCNA、Survivin蛋白表达的影响

目的：观察选择性环氧合酶-2（cyclooxygenase-2，COX-2）抑制剂尼美舒利对人胆管癌细胞系QBC939生长的抑制作用及Survivin基因表达的变化。方法：应用MTT比色法、细胞群体倍增时间观察尼美舒利对人胆管癌细胞QBC939增殖的影响，流式细胞仪检测细胞凋亡，免疫组织化学法观察尼美舒利对QBC939细胞PCNA，Survivin蛋白表达的影响。结果：尼美舒利呈时间、剂量依赖性抑制胆管癌增殖，高浓度（200μmol／L）尼美舒利不仅抑制胆管癌细胞增殖，而且诱导其凋亡。流式细胞仪研究显示，随药物浓度增加，细胞凋亡率显著增加。免疫组化结果显示尼美舒利处理后的QBC939细胞PCNA，Survivin蛋白的表达明显减弱。结论：尼美舒利能抑制QBC939细胞增殖，诱导其凋亡，其机制可能与PCNA，Survivin蛋白的表达下调有关。

Effects of nimesulide on proliferation and PCNA, Survivin protein expression on cholangiocarcinoma cells

Objective：To observe the inhibitory effects of a selective inhibitor nimesulide on growth of human cholangiocarcinoma cell line QBC939 in vitro and Survivin gene expression induced by nimesulide. Methods： The inhibitive effect of nimesulide on QBC939 was measured with MTT and TD. Apoptosis of QWBC 939 was measured by flow cytometry. The expression of PCNA and Survivin protein was determined by immunohistochemical analysis. Results： Nimesulide showed a dose - dependent and time - dependent growth inhibition on QBC939 cells. High concentration of nimesulde （200μmoL/L） not only inhibited the growth of QBC939 cells but also induced apoptosis. Flow cytometry showed that the apoptotic rates of QBC939 cells increased significantly as the dose of nimesulide increased. Condusion：Nimesulide can inhibit the proliferation of QBC939 cells, possibly by down - regulating the PCNA and Survivin protein.