生长激素释放肽对心衰大鼠植物神经重构的影响

本研究观察了生长激素释放肽(GHRP)的应用对心衰动物胆碱能神经和肾上腺素能神经支配的影响。Wistar大鼠50只,随机分为:假手术组10只;GHRP组30只;模型组10只。心衰模型成功后4周,Karnovsky-Roots法及免疫组织化学方法分别显示心肌胆碱能神经纤维及肾上腺素能免疫阳性纤维,应用多功能真彩色病理图像分析系统分析两种神经纤维密度。结果显示,模型组大鼠心肌中胆碱能神经纤维和肾上腺素能神经纤维密度明显低于假手术组(P<0.01);GHRP组大鼠心肌中两种神经纤维密度较模型组明显增高(P<0.01),但略低于假手术组神经纤维密度(P<0.05)。上述研究表明GHRP可延缓大鼠心衰后的去神经支配,改善神经重构。

Effect of growth hormone-releasing peptide on autonomic nerves' reconstitution after rat heart failure

We observed the effect of growth hormone-releasing peptide(GHRP)on cholinergic nerve fibers and adrenergic nerve fibers after heart failure.Fifty Wistar rats were divided into 3 groups:the false surgery group(n=10);GHRP group(n=30);model group(n=10).The samples were collected 4 weeks after heart failure and then the cholinergic nerve fibers were labeled with Karnovsky-Roots method;the adrenergic nerve fibers were stained with immunohistochemistry.The densities of those two nerve fibers were analyzed by using the analysis of multi-function color pathological image system.The results showed that the densities of cholinergic nerve fibers and adrenergic nerve fibers in the model group were obviously lower than that in the false surgery group(P<0.01);the densities of those two nerve fibers in the GHRP group were higher than that in the model group(P<0.01),but a bit lower than that of the false surgery group(P<0.05).These results suggest that GHRP can delay the denervation and ameliorate the nerve reconstitution of heart failure in rats.