中国北方汉族男性血管内皮生长因子基因C936T单核苷酸多态性与HiHiLo后心功能变化的关联研究

目的:探讨中国北方汉族男性血管内皮生长因子(VEGF)基因C936T单核苷酸多态性与HiHiLo后心功能变化的关联性。方法:选取62名中国北方平原地区汉族男性进行为期30天高住高练低训(HiHiLo),每日在低氧房(O2浓度为14.8%～14.3%,海拔约2800～3000米)休息10小时,每周进行3次75%VO2max强度的低氧训练(O2浓度为15.4%～14.8%,海拔约2500～2800米),运动时间为30mim/次,其余时间在常氧环境下训练。使用Doppler超声心动图仪测试HiHiLo前后左心室结构和功能。采用PCR-RFLP法分析VEGF基因C936T单核苷酸多态性。结果:VEGF基因C936T多态性与HiHiLo后的LVM、LVMI、SV以及SI的变化有关联。其中,CC基因型LVM、LVMI、SV及SI增加幅度均显著高于CT基因型。结论:中国北方汉族男性VEGF基因C936T多态性的CC基因型者在HiHiLo训练后产生了更好的心脏适应性变化,可以作为预测HiHiLo后部分心功能(SV、SI)变化的分子遗传学标记。

Association between VEGF Gene Polymorphism and Changes in Left Ventricular Function after HiHiLo Training in Men of Han Nationality from Northern China

Objective To explore the association between VEGF gene polymorphism and the changes in cardiac function after HiHiLo. Methods Sixty two men of Han nationality from northern China underwent HiHiLo for 30 days. Training programme was as follows:exposure in hypoxic environment (14.8% - 14.3%O_2) 10hrs per day, hypoxic training three times per week (15.4%～14.8%O_2), and training at sea level in the remaining time. The genotype was analyzed by PCR-RFLP. Results There is an association between VEGF gene polymorphism and the changes in left ventricular structure and function after 4-week HiHiLo. LVM, LVMI, SV and SI after HiHiLo increased significantly in men carrying CC genotype were better than those carrying CT genotype. Conclusion Men of Han nationality carrying CC genotype probably have a better left ventricular function in response to the HiHiLo than those carrying CT genotype. CC genotype can be used as a genetic marker for the better responsiveness of left ventricular function to the HiHiLo.