PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值

目的：探讨PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值。方法：对1486例疑为泌尿生殖道感染患者分别采用PCR反向膜杂交法及荧光定量PCR（FQ—PCR）检测，并将检测结果与培养结果比较。结果：FQ—PCR检测CT、UU、NG阳性率高于培养法，比较差异有统计学意义（P〈0．01）；PCR反向膜杂交法与培养法及FQ—PCR检测阳性率比较差异均无统计学意义；PCR反向膜杂交法、FQ—PCR检测敏感性比较差异无统计学意义，但PCR反向膜杂交法检测特异性高于FQ—PCR，比较差异有统计学意义（P〈001）。结论：PCR反向膜杂交法筛查CT、UU、NG准确，快速，且筛查特异性高于F0-PER，值得临床推广适用。，

PCR Reverse Hybridization Membrane Applications in Screening of CT,UU, NG Three Kinds of STD

Objective： To investigate the value of PCR reverse hybridization membrane application in screening of CT, UU, NG three kinds of STD. Method： 1486 suspected patients with genitourinary tract infections were delected by PCR reverse hybridization membrane and fluorescence quantitative PCR （ FQ-PCR ）, and test results were compared with culture results. Result： FQ-PCR detection of CT, UU, NG was higher than culture （ P〈0.01 ）. PCR reverse hybridization membrane, culture and FQ-PCR positive rate difference was not statistically significant. PCR reverse hybridization membrane, FQ-PCR detection sensitivity difference was not statistically significant, but the film PCR reverse hybridization assay specificity was higher than FQ-PCR （ P〈0.01 ） . Conclusion： PCR reverse hybridizat：ion in screening of CT, UU, NG, is accurate, rapid, and specific is higher than FQ-PCR, worthy of promotion apply.