| 重组人SOD工程菌发酵表达条件的优化 |
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| 引用本文： | 刘建荣,宋苍苍. 重组人SOD工程菌发酵表达条件的优化[J]. 中国医药工业杂志, 2011, 42(7): 501-503,A64 |
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| 作者姓名： | 刘建荣 宋苍苍 |
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| 作者单位： | 河北大学生物工程技术研究中心,河北保定,071002 |
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| 基金项目： | 河北省教育厅科学研究计划项目(2006308); 河北省科技研究与发展指导计划项目(06276455); 河北省生物工程重点学科资助项目(2008011) |
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| 摘 要： | 采用正交试验优化重组人超氧化物歧化酶工程菌的LB培养基,得到培养基最佳配比(g/L):蛋白胨12,酵母膏5,葡萄糖1和氯化钠10.以此为基础优化基因工程菌发酵条件,确定培养基初始pH 6.5～7.5,装液量20％,菌体密度(D600) 0.55～1.4,42℃诱导表达2～3h,目的蛋白相对表达量由优化前的25％提高至4...
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| 关 键 词： | 重组人超氧化物歧化酶 基因工程菌 发酵 诱导 表达 |
Optimization of Fermentation Conditions of Recombinant Human Superoxide Dismutase Genenic Engineering Bacteria |
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| LIU Jianrong,SONG Cangcang. Optimization of Fermentation Conditions of Recombinant Human Superoxide Dismutase Genenic Engineering Bacteria[J]. , 2011, 42(7): 501-503,A64 |
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| Authors: | LIU Jianrong SONG Cangcang |
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| Affiliation: | LIU Jianrong,SONG Cangcang (Biotechnological Research Center,Hebei University,Baoding 071002) |
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| Abstract: | The LB medium of recombinant human superoxide dismutase genenic engineering bacteria was optimized by orthogonal test.The optimal ratios of medium components(g/L) were as follows:peptone 12,yeast extract 5,glucose 1 and sodium chloride 10.The fermentation conditions were optimized in the above optimal medium.The relative expression rate of rhSOD was increased from 25%to 41%,and the absolute expression rate of rhSOD was found 2.4 times as that before optimization when the incubation parameters were adjusted ... |
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| Keywords: | rhSOD genetic engineering bacteria fermentation induction expression |
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