口服透明质酸在大鼠体内吸收机制的研究

 目的探讨透明质酸口服吸收的机制。方法采用放射免疫法测定大鼠单剂量口服透明质酸溶液和3种单糖(D-葡糖 醛酸、N-乙酰氨基葡糖及两者等摩尔配比)溶液后血清中透明质酸的浓度,用梯形法计算AUC。结果 生理盐水对照组和D-葡糖醛酸组的血清中透明质酸没有出现峰值;透明质酸组在口服后约2 h,出现明显的透明质酸吸收峰;另外两个单糖组在口 服后7 h左右,出现一个明显的透明质酸合成峰。透明质酸组的AUC_0～13 h为2 417μg·h·L^-1,实测ρ_max为187．6μg·L^-1,t_max为 2．0 h。结论透明质酸口服后可以被机体吸收,且部分能以非单糖的形式被机体吸收。

Study on absorption of hyaluronic acid after an oral administration in rats

OBJECTIVE To study the absorption of hyaluronic acid (HA) administrated orally in rats.METHODS The concentrations of HA in rats serum were determined by the radioimmumoassay after a single dose of HA and three monosaccharides (D-glucuronic acid,N-acetylglucosamine and the equal mole ratio of both) were administered orally. The AUC was calculated by trapezoidal integration. RESULTS There were no evident changes with the serum HA levels in both of saline group and D-glucuronic acid group. The serum HA levels in HA group appeared an absorption peak at 2 h after an oral administration. The serum HA levels of other monosaccharides groups appeared a synthetic peak of HA at about 7 h after an oral administration. The main pharmacokinetic parameters of HA were as follows: AUC_0～13 2 417 μg·h·L^-1,ρ_max 187.6 μg·L^-1, t_max 2.0 h.CONCLUSION HA is absorbed and partial HA is absorbed as the form of non-monosaccharide after an oral administration.