| 脂肪基质干细胞跨胚层分化为神经干细胞的实验研究 |
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| 引用本文： | 吴雷,陈若琨,杨靓,杨转移,柴志勇,方加胜.脂肪基质干细胞跨胚层分化为神经干细胞的实验研究[J].第四军医大学学报,2009,30(1):70-72. |
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| 作者姓名： | 吴雷 陈若琨 杨靓 杨转移 柴志勇 方加胜 |
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| 作者单位： | 中南大学湘雅医院神经外科,湖南,长沙,410008
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| 摘 要： | 目的：探讨脂肪基质干细胞向神经干细胞跨胚层分化的可行性，进而为神经系统损伤修复寻找理想的种子细胞．方法：无菌条件下取大鼠腹股沟脂肪组织，消化离心后低糖含血清培养基培养，待细胞生长至亚融合状态改用神经干细胞培养基诱导，用免疫荧光对诱导的细胞进行鉴定．结果：大鼠脂肪基质干细胞在相应培养条件下快速分裂增殖为大而圆并含粗大胞质颗粒的细胞，后者形成细胞球（或称“神经球”），该细胞球表达特殊的神经干细胞Nestin抗原；若进一步将这些细胞球分离成单细胞并重新以克隆密度培养，单个的细胞又很快形成新的神经球．神经干细胞球进一步分化，可见到有的细胞胞体增大并出芽，逐渐发育成为较成熟的长突起细胞，长突起相互连接、交织成网并建立有神经纤维样联系，表达Nse或Gfap抗原．结论：大鼠脂肪基质干细胞在一定条件下可以向神经干细胞跨胚层分化．该种来源的神经干细胞可能成为中枢神经系统损伤修复的理想种子细胞．
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| 关 键 词： | 脂肪基质干细胞 神经干细胞 细胞分化 细胞培养 |
Differentiation of adipose-derived stem cells into nerve stem cells across embryonic layer |
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| WU Lei,CHEN Ruo-Kun,YANG Liang,YANG Zhuan-Yi,CHAI Zhi-Yong,FANG Jia-Sheng.Differentiation of adipose-derived stem cells into nerve stem cells across embryonic layer[J].Journal of the Fourth Military Medical University,2009,30(1):70-72. |
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| Authors: | WU Lei CHEN Ruo-Kun YANG Liang YANG Zhuan-Yi CHAI Zhi-Yong FANG Jia-Sheng |
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| Institution: | WU Lei,CHEN Ruo-Kun,YANG Liang,YANG Zhuan-Yi,CHAI Zhi-Yong,FANG Jia-ShengDepartment of Neurosurgery,Xiangya Hospital,Central South University,Changsha 410008,China |
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| Abstract: | AIM:To explore the feasibility of differentiation of adipose-derived stem cells (ADSCs) into neural stem cells (NSCs), so as to search the perfect source of seed cells to repair the tissue injury of the central nervous system. METHODS: ADSCs, derived from the adipose of rat's groin, were induced in NSC-culture medium, and then the differentiated cells were identified with immunofluorescence. Proliferation of NSCs was evaluated by formation of cell clones. Antibodies against Nestin, NSE and GFAP were used fo... |
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| Keywords: | adipose-derived stem cells neural stem cells cellular differentiation cell culture |
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