原花青素对氨基脲致雄性小鼠生殖毒性的拮抗作用

目的观察原花青素(PC)对氨基脲(SEM)致雄性小鼠生殖毒性的拮抗作用及作用机制。方法 50只KM雄性小鼠随机等分为5组:溶剂对照组、SEM染毒组、低、中、高剂量PC保护组。溶剂对照组灌胃去离子水;SEM染毒组先按56.25 mg/kg.bw SEM溶液灌胃染毒2周,再继续灌胃去离子水4周;3个剂量PC保护组,先分别灌胃PC 100、200、400 mg/kg.bw 4周,继续分别灌胃同剂量SEM溶液2周;以上各组动物均按10 ml/kg.bw体积灌胃每日1次,连续灌胃6周剖杀,取其血液和组织器官检测生殖相关指标。结果 PC保护组睾丸形态较SEM染毒组有所改善,且随PC剂量的加大改善较明显;低、中、高剂量PC组小鼠精子数、精子活动度较SEM组增加,畸形率降低;血清T、LH、FSH含量均高于SEM染毒组,SOD、GSH-PX活力上升、MDA、GSH含量下降与SEM染毒组比较,差异均有统计学意义(P<0.05)。结论适量PC可保护SEM对雄性小鼠亚慢性生殖系统的损伤;抗氧化损伤是PC保护SEM致生殖损伤的重要机制。

Antagonism of Procyanidins on Semicarbazide-induced Reproductive Toxicity in Male Mice

Objective To observe the antagonism and its mechanism of procyanidins(PC) on semicarbazide(SEM) caused reproductive toxicity in male mice. Methods Fifty KM male mice were randomly divided into 5 groups: solvent control group,SEM exposure group,low-,medium-and high-dose PC protection groups.The mice of solvent control group were administrated intragastrically with deionized water.The mice of SEM exposure group were administered intragastrically with 56.25 mg/kg.bw SEM solution in the first two weeks,and then were dosed by gavage with deionized water for 4 weeks.The mice of three doses groups were fed with PC 100,200 and 400 mg/kg.bw separately in the first four weeks,and then fed with the same dose of SEM solution for the next two weeks separately.All animals in each group received intragastric administration once a day according to 10 ml/kg.bw volume.After 6-week oral administration,the mice were sacrificed and the reproductive related indicators of blood,tissues and organs were detected. Results Testicular morphology in PC protection group was improved compared with SEM exposure group,and the improving effect was more obvious with the increasing dose of PC.Compared with SEM exposure group,sperm count and activity of the mice in low-,medium-and high-dose PC groups were increased significantly,while the malformation rates were decreased.Serum levels of T,LH,and FSH in the three doses groups were all higher than those of SEM exposure group.Compared with SEM exposure group,SOD and GSH-PX activity of the three doses groups increased,while MDA and GSH content decreased,and the difference was statistically significant(P<0.05). Conclusions The addition of proper amount of PC has protection effects on the reproductive system of sub-chronic injury in male mice.Antioxidation is an important mechanism of PC in protecting from SEM-induced reproductive damage.