降钙素基因相关肽刺激体外培养脊髓神经元细胞c-fos的表达

背景：内脏神经敏感性增高是胃肠道功能性疾病的重要发病机制之一，降钙素基因相关肽（CGRP）可能是其中重要的神经递质。目的：通过c-fos测定证实CGRP在脊髓内具有神经递质功能。方法：制备并培养大鼠脊髓神经元细胞，分别加入不同剂苗（10、20和40μl 100μmol／L）的CGRP，或经CGRP受体拮抗剂hCGRP8-37预处理后再加CGRP，行c-fos免疫组化染色和荧光定量聚合酶链反应（PCR）。结果：加入CGRP后，脊髓神经元的c-fos表达明显增加，但该作用可被CGRP受体挂号抗剂hCGRP8-37所阻断：c-fos的表达量与CGRP的浓度相关。结论：CGRP能激活脊髓神经元。

Calcitonin Gene-related Peptide Induces c-fos Expression in Rat Spinal Neuron in vitro

Background: Visceral hypersensitivity is one of the most important mechanisms of functional gastrointestinal diseases and calcitonin gene-related peptide (CGRP) may be the important neurotransmitter involved. Aims: To confirm that CGRP is a neurotransmitter that can activate spinal neuron. Methods: Experiments were conducted on primary cultures of neonatal rat spinal neurons. CGRP of diverse concentrations and its receptor antagonist hCGRP8-37 were added to the cell cultures, respectively. c-fos expression was detected by immunohistochemistry and fluorescent quantitation polymerase chain reaction(PCR). Results: Treatment of cultures with CGRP for 2 h increased c-fos mRNA expression; and CGRP antagonist hCGRP8-37 can block CGRP expression. The CGRP-induced change in c-fos expression was concentration-dependent. Conclusions: CGRP can activate spinal neuron cultured in vitro.