| 端粒酶反义腺病毒载体对乳腺癌细胞MCF-7端粒酶的抑制作用 |
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| 作者姓名： | Zhang X Tong T |
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| 作者单位： | [1]北京医科大学化与分子生物学系 [2]北京医科大学生与分子生物学系 |
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| 基金项目： | 国家自然科学基金资助课题(39930170)和国家重点基础研究发展规划(G1999053906)资助课题 |
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| 摘 要： | 端粒酶ＲＮＡ的反义地人乳腺癌细胞系ＭＣＦ－７细胞端粒酶活性的影响。方法用重组腺病毒转移并表达端粒酶ＲＮＡ的反义ｃＤＮＡ,采用基因重组腺脂质体共转当闰酶反义重组病毒,用Ｓｏｕｔｈｅｒｎ杂交鉴定病毒的整合功能,用ＴＲＡＰ－ 染法检测端粒酶活性。结果ＭＣＦ－７细胞是恶性乳腺癌的典型细胞系。对对照组ＭＣＦ－７、ＭＣＦ－７、ｖＡｄ－ＡＡＶ细胞相比,反义病毒感染后的细胞是恶性乳腺癌的典型细胞系。与对照组ＭＣＦ
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| 关 键 词： | 乳腺癌 端粒 端粒酶 RNA MCF－7 基因抑制 |
| 修稿时间： | 1999-07-26 |
The inhibitory effect of antisense adenovirus vector of telomerase on telomerase activity in breast cancer cells MCF-7 |
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| Zhang X,Tong T.The inhibitory effect of antisense adenovirus vector of telomerase on telomerase activity in breast cancer cells MCF-7[J].Chinese Journal of Pathology,2000,29(3):188-191. |
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| Authors: | Zhang X Tong T |
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| Institution: | Email:biochem@mail.bjmu.edu.cn |
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| Abstract: | Objective To inhibit telomerase activity in breast cancer cells MCF 7 by antisense ribonucleotide technique. Methods Recombinant adenovirus was chosen as gene delivery vector to transfer and express the antisense RNA component of telomerase in tumor cells. The recombinant adenovirus of anti telomerase RNA was obtained through gene recombination and co transfection with lipofectin. The integrated potential of antisense virus was certified by Southern blot and the telomerase activity of MCF 7 was analysed by TRAP silver staining. Results The MCF 7 cell line is a typical model for malignant breast cancer. In this study, the telomerase activity of MCF 7 was significantly inhibited after antisense virus infection when compared with the control cell lines. Conclusion The introduction of antisense cDNA of telomerase RNA can significantly inhibit the activity of telomerase in MCF 7 cells. |
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| Keywords: | Breast neoplasms Telomerase Adenoviruses human Gene suppressor tumor |
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