大孔吸附树脂分离纯化消癥丸提取液工艺研究

目的研究消癥丸提取液分离纯化工艺。方法以总皂苷、总蒽醌、阿魏酸、橙皮苷比上柱量、比吸附量、比洗脱量作为考察指标,采用Z值综合评分法,优选消癥丸提取液纯化工艺。结果 AB-8型大孔树脂对以上4类成分具有良好的吸附效果,静态上样为最佳上样工艺,最佳工艺参数为提取液质量浓度0.5 g/m L,浸泡时间24 h,柱径高比1∶10,水除杂用量14 BV(BV为柱体积),80%乙醇洗脱,洗脱剂用量10 BV,洗脱体积流量2 BV/h。结论 AB-8型大孔树脂分离纯化消癥丸提取液的方法可行。

Research on separation and purification of extract in Xiaozheng Pills with macroporous resins

Objective To explore the optimal separation and purification of extract in Xiaozheng Pills (EXP). Methods We chosed saturation ratio, adsorption ratio, and desorption ratio of total saponins, total anthraquinone, ferulic acid, and hesperidin as evaluation indexes to optimize the purification process of EXP using Z score method. Results Macroporous resin AB-8 had the best adsorption and desorption properties, while static adsorption was superior to dynamic adsorption. In the course of adsorption, the optimum concentration of the sample liquid was 0.5 g/mL equivalent to raw materia1, static adsorption of 24 h and the resin column diameter-height ratio of 1:10. In the course of elution, the resin column chromatography was eluted with 10 BV of 80% EtOH after removing impurities with 14 BV of water by flow rate of 2 BV/h. Conclusion Macroporous resin AB-8 is suitable to separate and purify EXP.