脊髓全横断大鼠模型的构建☆

背景：脊髓全横断模型在造模时常难以保证神经纤维的完全离断。 目的：构建大鼠脊髓全横断损伤模型。 方法：将大鼠随机分为模型组和假手术组。模型组构建脊髓T10节段全横断模型；假手术组动物仅打开椎管与硬脊膜而后缝合，但不损伤脊髓。建模后1，3，5，7 d分别进行BBB评分以评估后肢运动功能，检测其体感诱发电位和运动诱发电位来评估神经传导通路的完整性，并行形态学观察来评估脊髓肉眼观病理形态。 结果与结论：与假手术组相比，模型组大鼠在建模后1，3，5，7 d时，其BBB评分降低(P < 0.01)，未检测出体感和运动诱发电位。形态学观察结果显示模型组大鼠脊髓完全横断，而假手术组脊髓形态完整。结果提示实验成功构建了大鼠脊髓全横断模型。

Establishment of spinal cord transection injury models in rats

BACKGROUND: The spinal cord injury (SCI) models reported in literature were short of determinacy and reliability. OBJECTIVE: To establish an accurate and reliable rat spinal cord transection injury models. METHODS: Rats were divided into model and sham-surgery groups at random. Model group prepared for T10 transection injury models; vertebral canal and spinal dura mater were exposed and sutured in the sham-surgery group. The motor function of hind limbs was evaluated by Basso, Beattie and Bresnahan (BBB) scores; the integrality of the nervous pathways was detected by somato-sensory evoked potential (SEP) and motor evoked potential (MEP), in addition, the pathologic and gross pathology of spinal cord were performed at 1, 3, 5 and 7 days after modeling. RESULTS AND CONCLUSION: Compared with the sham-surgery group, the BBB scores of the model group were decreased at 1, 3, 5 and 7 days after modeling (P < 0.01). The SEP and MEP were not evoked out in the model group. The spinal cords were fully transected in the model group, which was intact in the sham-surgery group. that of. The result suggested that a spinal cord transection injury model is established successfully.