含母牛分枝杆菌菌苗凝胶肺内应用的免疫效应研究

目的 观察含母牛分枝杆菌菌苗(微卡)凝胶在肺部介入应用后产生的局部及全身免疫应答效应.方法 60只日本长耳兔随机分成5组,每组12只.第1组为对照组,气管插管至末梢支气管注入生理盐水3 ml;第2组为单纯凝胶组,肺部注入卡波姆凝胶3 ml;第3、4、5组为微卡凝胶组,分别注入含3.75、7.50、9.37 mg/L母牛...

Study on immune response of Mycobacterium vaccae gel administered in lung

Objective To observe local and systemic immunity effect of Mycobacterium vaccae gel administered in lung.Methods 60 Japanese long-eared rabbits were randomly divided into five groups.Group 1 was injected with 3 ml normal sodium.Group 2 was injected with 3 ml carbomer gel.Group 3,4,5 were injected with 3 ml carbomer gel with 3.75,7.50,9.37 mg/L Mycobacterium vaccae.The peripheral blood T cell subsets and interferon-γ were tested.Results Before sacrificed,CD3＋ percents in group 4 and group 5 [（55.8±9.7）% and（54.5±11.8）%] were higher than that in group 1 [（43.1±3.0）%]（all P〈0.05）,CD4＋ percents in group 4 and group 5 [（44.1±5.8）% and（43.3±6.2）%] were higher than that in group 1 [（33.5±3.1）%]（all P〈0.05）,CD8＋ percents in group 3,4,5 [（21.3±3.3）%,（21.1±4.5）% and（20.5±4.1）%] were lower than that in group 1 [（28.2±1.7）%]（all P〈0.05）.Interferon-γ was positive in the lung tissues injected with carbomer gel with Mycobacterium vaccae in group 4 and group 5.Conclusions Local immune stimulation can cause systemic immune response in healthy Japanese long-eared rabbits.