Quantification of serum free light chain kappa and lambda by the SPAPLUS analyser |
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| Authors: | Diane Maisin Thibault Lepoutre Damien Gruson Pierre Wallemacq |
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| Affiliation: | 1. Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA;2. Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA;3. Department of Molecular & Experimental Medicine, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA;4. The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA;1. Tampere University Hospital, Critical Care Medicine Research Group, PO Box 2000, 33521 Tampere, Finland;2. Helsinki University Central Hospital, Lohja Hospital, Emergency Department, Sairaalatie 8, 08200 Lohja, Finland;3. Helsinki University Hospital, Department of Surgery, Division of Intensive Care Medicine, PO Box 340, 00029 Helsinki University Hospital, Finland;4. Kuopio University Hospital, Centre for Prehospital Emergency Care, PO Box 100, 70029 Kuopio University Hospital, Finland;5. Helsinki University Hospital, Jorvi Hospital, Department of Intensive Care, PO Box 800, 00029 Helsinki University Hospital, Finland;6. Helsinki University Hospital, Department of Neurology, PO Box 340, 00029 Helsinki University Hospital, Finland;7. University of Tampere, School of Health Sciences, 33014 University of Tampere, Finland;8. Kanta-Häme Central Hospital, Department of Intensive Care, Ahvenistontie 20, 13530 Hämeenlinna, Finland;9. South-Carelia Central Hospital, Department of Intensive Care, Valto Käkelänkatu 1, 53130 Lappeenranta, Finland;10. Lapland Central Hospital, Department of Intensive Care, PL 8041, 96101 Rovaniemi, Finland;11. Uppsala University Hospital, Department of Surgical Sciences, 75185 Uppsala, Sweden;1. Department of Blood and Marrow Transplant and Cellular Immunotherapy, Moffitt Cancer Center, Tampa, FL;2. Program for Comparative Effectiveness Research, University of South Florida College of Medicine, Tampa, FL;3. Division of Hematology-Oncology and Blood and Marrow Transplantation Program, Mayo Clinic, Jacksonville, FL |
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| Abstract: | ObjectiveClinical assessment of the SPAPLUS® system for the determination of the serum free light chains kappa (κ FLC) and lambda (λ FLC) compared to the BNII®.Design and methods126 serum specimens from our routine activity were analysed on two different analysers: the BNII® (immunonephelometry, Siemens) and the SPAPLUS® (turbidimetry, Binding Site). We compared the absolute values of the serum κ FLC and λ FLC, as well as the FLC κ/λ ratio on both analysers. These results were further evaluated together with the clinical history of the patients.ResultsRegression analysis between the BNII® and the SPAPLUS® for κ FLC and λ FLC did not display any significant differences between both methods in the normal and pathological ranges. Nevertheless, some differences have been observed for some patients in the absolute value of the involved light chain, with potential clinical implications.ConclusionThe results show overall good concordance between both methods. However, it is recommended that the monitoring of patients affected by monoclonal gammapathies by measuring FLC, be performed in the same laboratory and by the same method. Moreover, the FLC results should always be interpreted together with other laboratory tests taking into account the patient's diagnosis. |
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