Biofilm formation by oral clinical isolates of Candida species |
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| Authors: | Luis Octavio Sánchez-Vargas Deyanira Estrada-Barraza Amaury J Pozos-Guillen Raimundo Rivas-Caceres |
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| Institution: | 1. Oral Microbiology, Pathology and Biochemical laboratory, Faculty of Stomatology, University Autonomous of San Luis Potosí, Mexico;2. Biomedical Sciences Institute, University Autonomous of Juarez City, Chihuahua, Mexico;3. Basic Science laboratory, Faculty of Stomatology, University Autonomous of San Luis Potosí, Mexico;4. Department of Chemical-Biological Sciences. Biomedical Sciences Institute, University Autonomous of Juarez City, Chihuahua, Mexico;1. Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC;2. Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC;3. Graduate Institute of Molecular Systems Biomedicine, China Medical University, Taichung 40402, Taiwan, ROC;1. CEB - Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, 4710-057 Braga, Portugal;2. Tissue Engineering & Reparative Dentistry, School of Dentistry, Cardiff University, Cardiff CF14 4XY, United Kingdom;1. Department of Oral Microbiology and Immunology, College of Dentistry, Republic of Korea;2. Department of Prosthodontics, College of Dentistry, Dankook University, Cheonan, Republic of Korea;1. Dumlupinar University Medical Faculty, Microbiology and Clinical Microbiology, Kutahya, Turkey;2. Dumlupinar University Medical Faculty, Department of Nephrology, Kutahya, Turkey;3. Mevlana University Medical Faculty, Department of Nephrology, Konya, Turkey;4. Y?ld?r?m Beyaz?t University Medical Faculty,Microbiology and Clinical Microbiology, Ankara, Turkey;1. Islamic Azad University, Borujerd Branch, Departmant of Microbiolgy, Borujerd, Iran;2. Lorestan University of Medical Sciences, Razi Herbal Medicines Research Center, Khorramabad, Iran;3. Islamic Azad University, Borujerd Branch, Department of Laboratory Sciences, Borujerd, Iran;4. Lorestan University of Medical Sciences, Faculty of Medicine, Department of Community Medicine, Khorramabad, Iran |
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| Abstract: | We have conducted a longitudinal study to quantify biofilms in oral clinical isolates of Candida species (spp.) from adults with local and systemic predisposing factors for candidiasis. A total of 69 yeast isolates from 63 Mexican patients were evaluated. These isolates (39 C. albicans, 15 C. tropicalis, 7 C. glabrata, 4 C. krusei, 1 C. lusitaniae, 1 C. kefyr, 1 C. guilliermondii and 1 C. pulcherrima) were obtained from two clinical sites: 62.3% (n = 43) from the oral mucosa of totally and partially edentulous patients, and 37.7% (n = 26) from the oral mucosa of diabetics. In addition, Candida ATCC strains were used as controls for each experiment. The kinetics of biofilm formation were measured by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)carbonyl]-2H-tetrazolium hydroxide XTT] reduction; each isolate was tested at 6, 12 and 24 h. Biofilm formation is dependent on the Candida spp. and its clinical origin. On average, the oral isolates of C. glabrata are strong biofilm producers, whereas C. albicans and C. tropicalis are moderate producers. The most common species in our population was C. albicans. While the kinetics of C. albicans biofilm formation varies between oral isolates, it generally maintains steady growth from 2 to 48 h, when it reaches its maximum growth. |
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| Keywords: | Biofilm Clinical isolates Diabetes mellitus 2 Denture wearers |
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