Curcumin reduces Streptococcus mutans biofilm formation by inhibiting sortase A activity |
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| Authors: | Ping Hu Ping Huang Min Wei Chen |
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| Institution: | 1. Department of Operative Dentistry and Endodontics, School of Stomatology, Fourth Military Medical University, Xi’an, China;2. Department of General Surgery, The 456th Hospital of PLA, Ji’nan City, Shandong, China;3. Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China;1. Endodontics Unit, Section of Dental Medicine, University of Geneva, 19 rue Barthelemy Menn, CH-1205 Geneva, Switzerland;2. Flow Cytometry Facility, Faculty of Medecine, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland;3. Department of Pharmaceutics and Biopharmaceutics, School of Pharmaceutical Sciences, University of Geneva, Quai Ernest Ansermet 30, CH-1211, Geneva 4, Switzerland;4. Service of Infectious Diseases, University Hospitals of Geneva, Rue Micheli-du-Crest 24, CH-1205 Geneva, Switzerland;5. Department of Restorative Dentistry, University of Washington, Box 357456, 1959 NE Pacific Street, Seattle, WA 98195-7456, USA;1. Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Brazil;2. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Brazil;1. Department of Pedodontics, Jundishapur University of Medical Sciences, Ahvaz, Iran;2. Student Research Committee, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran;3. Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;4. Department of Orthodontics, Jundishapur University of Medical Science, Ahvaz, Iran;1. Department of Pediatric Dentistry, Araraquara Dental School, UNESP – Univ Estadual Paulista, Araraquara, SP 14801-903, Brazil;2. Araraquara Dental School, UNESP – Univ Estadual Paulista, Araraquara, SP 14801-903, Brazil;3. Department of Pathology and Physiology, Araraquara Dental School, UNESP – Univ Estadual Paulista, Araraquara, São Paulo 14801-903, Brazil;4. São Carlos Institute of Physics, University of São Paulo, USP, São Carlos, São Paulo, Brazil;1. Multidisciplinary Institute in Health, Federal University of Bahia – UFBA, Brazil;2. Institute of Physics of São Carlos, University of São Paulo, São Carlos, SP, Brazil |
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| Abstract: | BackgroundSortase A is an enzyme responsible for the covalent attachment of Pac proteins to the cell wall in Streptococcus mutans. It has been shown to play a role in modulating the surface properties and the biofilm formation and influence the cariogenicity of S. mutans. Curcumin, an active ingredient of turmeric, was reported to be an inhibitor for Staphylococcus aureus sortase A. The aim of this study was to investigate the inhibitory ability of curcumin against S. mutans sortase A and the effect of curcumin for biofilm formation.MethodsThe antimicrobial activity of the curcumin to the S. mutans and inhibitory ability of the curcumin against the purified sortase A in vitro were detected. Western-blot and real-time PCR were used to analysis the sortase A mediated Pac protein changes when the S. mutans was cultured with curcumin. The curcumin on the S. mutans biofilm formation was determined by biofilm formation analysis.ResultsCurcumin can inhibit purified S. mutans sortase A with a half-maximal inhibitory concentration (IC50) of (10.2 ± 0.7) μmol/l, which is lower than minimum inhibitory concentration (MIC) of 175 μmol/l. Curcumin (15 μmol/l) was found to release the Pac protein to the supernatant and reduce S. mutans biofilm formation.ConclusionsThese results indicated that curcumin is an S. mutans sortase A inhibitor and has promising anti-caries characteristics through an anti-adhesion-mediated mechanism. |
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| Keywords: | Inhibition Sortase A Curcumin |
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