| 溶血磷脂酸对肝癌细胞迁移行为的影响及其相关机制 |
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| 引用本文： | 林川川,罗庆,陈哲,宋关斌. 溶血磷脂酸对肝癌细胞迁移行为的影响及其相关机制[J]. 医用生物力学, 2016, 31(5): 390-395 |
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| 作者姓名： | 林川川 罗庆 陈哲 宋关斌 |
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| 作者单位： | 重庆大学 生物工程学院;重庆大学 生物工程学院;重庆大学 生物工程学院;重庆大学 生物工程学院 |
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| 基金项目： | 国家自然科学基金项目(11272365，11532004)，高等学校博士学科点专项科研基金项目（20130191110029），中央高校基本科研业务费资助项目（106112015CDJZR238807） |
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| 摘 要： | 目的研究溶血磷脂酸(lysophosphatidic acid,LPA)对肝癌细胞MHCC97H迁移行为的影响,并探究其相关的分子机制。方法采用Transwell法检测LPA处理后MHCC97H细胞迁移的变化情况,并通过Rho相关蛋白激酶(ROCK)抑制剂Y-27632检测ROCK信号通路在其中的作用,利用免疫荧光染色和免疫印迹法检测LPA对MHCC97细胞骨架F-actin表达的影响,通过原子力显微镜检测LPA作用后MHCC97H细胞弹性模量的变化。结果LPA显著促进MHCC97H的迁移,ROCK抑制剂Y-27632可阻断LPA诱导的MHCC97H细胞迁移。LPA上调MHCC97H中F-actin的表达,减小MHCC97细胞的弹性模量。结论 LPA可能主要通过ROCK/F-actin通路降低肝癌细胞MHCC97H的硬度,从而促进其迁移行为。
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| 关 键 词： | 溶血磷脂酸； 肝癌细胞； 细胞迁移； 分子机制； 弹性模量 |
| 收稿时间： | 2016-04-08 |
| 修稿时间： | 2016-06-28 |
Effect of lysophosphatidic acid on hepatocellular carcinoma cell migration and its involved mechanisms |
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| LIN Chuan-chuan,LUO Qing,CHEN Zhe,SONG Guan-bin. Effect of lysophosphatidic acid on hepatocellular carcinoma cell migration and its involved mechanisms[J]. Journal of Medical Biomechanics, 2016, 31(5): 390-395 |
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| Authors: | LIN Chuan-chuan LUO Qing CHEN Zhe SONG Guan-bin |
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| Affiliation: | College of Bioengineering, Chongqing University;College of Bioengineering, Chongqing University;College of Bioengineering, Chongqing University;College of Bioengineering, Chongqing University |
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| Abstract: | Objective To investigate the effect of lysophosphatidic acid (LPA) on migration of hepatocellular carcinoma MHCC97H cells and its involved mechanisms. Methods Transwell was utilized to investigate the impact of LPA on cell migration of MHCC97H cells. Furthermore, the role of ROCK in the migration of MHCC97H cells through Y-27632 (a specific inhibitor of ROCK). Then, the expression of F-actin was observed with immunofluorescence staining and Western blotting. Atomic force microscopy (AFM) was employed to investigate elastic modulus of MHCC97H cells. Results LPA significantly promoted the migration of MHCC97H cells, while Y-27632 significantly blocked the migration of MHCC97H induced by LPA. Moreover, LPA up-regulated the expression of F-actin and decreased the elastic modulus of MHCC97H cells. Conclusions LPA promotes MHCC97H cell migration through decreasing the cell stiffness via ROCK/F-actin. |
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| Keywords: | Lysophosphatidic acid (LPA) Hepatocellular carcinoma cells (HCC) Cell migration Molecular mechanism Elastic modulus |
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