| 软骨细胞在异体脱细胞软骨基质上的体外培养实验 |
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| 引用本文： | 栾杰,蔡哲,杨佩瑛,宋业光,马海欢,唐勇,李艳妹,彭君,李丹. 软骨细胞在异体脱细胞软骨基质上的体外培养实验[J]. 中华烧伤杂志, 1999, 0(3) |
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| 作者姓名： | 栾杰 蔡哲 杨佩瑛 宋业光 马海欢 唐勇 李艳妹 彭君 李丹 |
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| 作者单位： | 中国医学科学院中国协和医科大学整形外科医院 100041北京 |
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| 摘 要： | 目的探讨脱细胞软骨基质对软骨细胞体外生长的影响。方法以脱细胞异体兔耳软骨基质为支架材料,以脱细胞异体兔耳软骨膜为对照,体外种植兔耳软骨细胞进行常规培养,观察软骨细胞的生长状况和增殖情况。结果软骨细胞进入脱细胞异体软骨基质构架裸露的空穴,生长旺盛。而在脱细胞异体软骨膜上,软骨细胞不易生长。结论支架材料的表面性状对细胞生长有显著影响,异体脱细胞软骨基质可提供适宜于软骨细胞生长的良好环境,有可能发展成为软骨组织工程的一种天然支架材料。
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| 关 键 词： | 组织工程学 软骨细胞 脱细胞软骨基质 细胞培养 |
Culture of chondrocytes using allogenous acellular cartilaginous matrix |
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| LUAN Jie CAI Zhe YANG Peiying et al.Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Beijing. Culture of chondrocytes using allogenous acellular cartilaginous matrix[J]. Chinese journal of burns, 1999, 0(3) |
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| Authors: | LUAN Jie CAI Zhe YANG Peiying et al.Plastic Surgery Hospital Chinese Academy of Medical Sciences Beijing |
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| Affiliation: | LUAN Jie CAI Zhe YANG Peiying et al.Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Beijing 100041 |
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| Abstract: | Objective To observe the effect of chondrocyte culture with natural ECM made from allogenous acellular cartilage.Methods The allogenous acellular cartilaginous matrix was used as a scaffold in chondrocyte cultrue in vitro,comparing with the acellular perichondrium.The growth and regeneration of the chondrocytes in vitro were observed.Results The chondrocytes seeded on the acellular cartilagi- nous matrix grew well while the chondrocytes on the acellular perichondrium matrix grew poorly.Conclu- sion The surface condition of the scaffold has a notable influence on the growth of the cells.The alloge- nous acellular cartilaginous matrix may provide a suitable condition for chondrocytes,and it has the possi- bility to be developed as a natural scaffold for tissue engineering. |
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| Keywords: | Tissue engineering Chondrocyte Acellular cartilaginous matrix Culture in vitro |
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