Rôle of a metabolite in allergenicity of Sudan I |
| |
Authors: | Shinobu Kato Hiroshi Itagaki Mariko Uchiyama Toshiaki Kobayashi Yoshio Fujiyama |
| |
Institution: | Laboratory of Toxicology and Dermatology, Shiseido Toxicological and Analytical Research Center, 1050 Nippa-cho, Knhokuku, Yokohama, Japan 223 |
| |
Abstract: | A metabolite of Sudan I (l-phenylazo-2—naphthol) was isolated alter in vitro incubation with the supernatant of guinea pig liver homogenate (S·9). The metabolite was found to be 4′-hydroxy-l-phenylazo-2—naplithol by gas chromatography-mass spectrormetry (GC-MS), and proton and carbon-13 nuclear magnetic resonance (NMR) analyses. It elicited positive reactions in guinea pigs sensitized ot Sudan I. It was also shown to be allergenic. The results suggest that para-hydroxylation of the phenyl group of Sudan I may play an important rôle in its allergenicity. |
| |
Keywords: | Contact allergenicity guinea pig Sudan I (l-Phenylazo-2—naphthol) 4—hydroxy-l-phenylazo-2 naphthol metabolite |
|