间日疟原虫MSP-1 和 CSP 基因遗传多样性的分析

目的比较间日疟原虫两种主要分子标志(MSP-1 和 CSP)的遗传多样性. 方法分别用MSP-1和CSP基因分型方法鉴定间日疟原虫现场分离株,并进行基因多态性比较和分析. 结果共检测32份海南省现场确诊的间日疟病人血样,MSP-1等位基因型混合感染率为18.75%,平均克隆数1.16;CSP基因型混合感染率为35.29%,平均克隆数为1.47.如果同时考虑两种基因型,混合感染率则为50.00%.空间对应分析发现,热带族与Sal-1型关系密切,PvⅡ型与重组Ⅲ型分布靠近,其他基因型则较分散. 结论同时用MSP-1和CSP两种分子标志检测间日疟原虫,其基因型混合感染率高于用单一标志检测,两种标志检测结果存在一定对应关系.

ANALYSIS ON PLASMODIUM VIVAX GENETIC DIVERSITY USING THE MARKERS OF MSP-1 AND CSP

Objective To study the genetic diversity of Plasmodium vivax by two main molecular markers(MSP-1 and CSP). Methods Isolates of P. vivax from Hainan malaria endemic area were collected and genotypes of MSP-1 and CSP were identified and compared. Results The alleles of MSP-1 and CSP from 32 blood samples of tertian malaria patients were identified. 18.75% and 35.29% multiple infections were observed in MSP-1 and CSP respectively. The mean clones were 1.16 and 1.47 respectively in the two genes. Combined the two markers, the multiple infection rate was 50%. The correspondence analysis on the alleles from the two different markers suggested that the genotype of Sal-1 in MSP-1 and the type of tropical zone family in CSP exhibited close relation, PvII type and Recombination III type had certain relation, no correspondence was observed in other types. Conclusion The multiple infection rate of genotypes is higher identified by combined two markers than by single one and a certain correspondence between the two MSP-1 and CSP molecular markers may exist.