大鼠视网膜缺血再灌注损伤过程中细胞凋亡的变化

目的在大鼠视网膜缺血再灌注损伤模型上,研究视网膜缺血再灌注损伤后视网膜内细胞凋亡的动态变化.方法30只大鼠随机分为6组,每组5只.通过结扎大鼠左颈总动脉1 h,然后再灌注,检测1、6、12、24、48、72 h各组大鼠视网膜内的细胞凋亡.结果再灌注后,细胞凋亡主要出现于视网膜的神经节细胞层和内核层,大鼠视网膜在再灌注6 h逐渐出现细胞凋亡,12 h逐渐增加,24 h细胞凋亡达到高峰,偶尔在外核层可见凋亡细胞.然后细胞凋亡逐渐减少,但是,到了术后72 h,仍然可见视网膜内有细胞凋亡.结论通过结扎颈总动脉,可以见到大鼠视网膜的缺血再灌注损伤,而细胞凋亡在视网膜缺血再灌注损伤中发挥着重要作用.到了手术后72 h,损伤的视网膜尚未完全恢复.

Apoptosis Changes in Rat Retina after Ischemia-reperfusion Injury

Objective To establish the rat ischemia -reperfusion injury model and learn the rat apoptosis changes after ischemia- reperfusion injury. Methods Ligate rat left common carotid artery 1 h, reperfuse and exam retinal apoptosis in 1,6, 12, 24, 48 and 72 h groups. Result Apoptosis mainly occurs in retina ganglion cell layer and inner nuclear layer, occasionally in outer nuclear layer. Apoptosis occurs in 6 h after reperfusion. In 24 h, apoptosis achieves the peak, then decrease gradually. However, in 72 h after reperfusion, there is apoptosis in rat retina. Conclusion rat retina ischemia- reperfusion injury can be observed after ligating common carotid artery. Apoptosis may play an important role in rat retina ischemia - reperfusion injury. In 72 h after the operation, the injured retina didnt recover completely.