清洁级BALB/c近交系小鼠常规生物学特性研究

目的建立BALB/c近交系小鼠常规生物学特性指标.方法采用日本光电MEK-5126血球计数仪、荷兰半自动生化仪、流式细胞仪及常规的实验方法检测.结果血液细胞计数、血液生化指标、体重、脏器重量、脏器指数等常规生物学指标结果比较均一;清洁级BALB/c近交系小鼠的血液细胞计数和血液生化指标同普通级BALB/c近交系小鼠比较差异显著;普通级的CD4^＋/CD8^＋指标雌雄之间及普通级与清洁级的CD4^＋/CD8^＋差异显著.结论清洁级BALB/c近交系小鼠具有比较稳定的生物学特性.

Study on Biological Parameters of Cleaning Inbred Strain BALB/c Mice

Objective To obtain the basic data of biological parameters of cleaning inbred strain BALB/c mice.Methods\ Routine methods and instruments were used to determine the biological parameters of 2 months old cleaning inbred strain BALB/c mice and immunological parameters of conventional inbred strain BALB/c mice.Results\ Similarities are shown in hematophysiological indices,biochemical indices,avoirdupois,weight of viscera,and index of viscera.Between cleaning BALB/c mice and conventional BALB/c mice,there are significant differences in hematophysiological indices and biochemical indices.On CD4~ /CD8~ indices,there are significant differences between male and female in conventional BALB/c mice,also between cleaning BALB/c mice and conventional BALB/c mice.Conclusion Biological parameters of cleaning inbred strain BALB/c mice are stable,owing to strictly controlled microorganisms and parasites;and some resulting influences on immunological function of mice.