Galactosylated chitosan-g-PEI/DNA complexes-loaded poly(organophosphazene) hydrogel as a hepatocyte targeting gene delivery system |
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Authors: | Hu-Lin Jiang You-Kyoung Kim Sun-Mi Lee Mi-Ran Park Eun-Mi Kim Yong-Mei Jin Rohidas Arote Hwan-Jeong Jeong Soo-Chang Song Myung-Haing Cho Chong-Su Cho |
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Institution: | 1. College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea 2. Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea 3. Division of Life Science, Korea Institute of Science and Technology, Seoul, 130-650, Korea 4. Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, 561-712, Korea
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Abstract: | Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system. |
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