肿瘤分化相关蛋白HIV-ep2/MIBP1/MBP2的结构及功能分析

目的预测和分析肿瘤分化相关蛋白HIV—ep2／MIBP1／MBP2的结构及功能。方法检索蛋白质全序列，应用所克隆的基因片段序列Genebank数据库中进行BLUST比对，得出HIV—ep2／MIBP1的全长序列。应用ExPASy提供的ProtParam程序对该蛋白质进行基本特征进行运算，进一步进行二级结构分析。SMART预测功能域，PDB网站模拟三维结构，ELM预测基元。蛋白质亚细胞定位的信息学预测；Source资源网站作组织表达分布。结果HIV—ep2／MIBP1／MBP2基因编码一个含有2500氨基酸的巨大蛋白质，属于不稳定蛋白。二级结构主要包括62．52％随机卷曲；22．36％α螺旋；10．32％伸展链；4．80％β转角。三级结构主要含有4个锌指结构，计算机预测了蛋白的三维立体结构，可能的基元46种。功能方面该蛋白定位于细胞核，在滤泡淋巴瘤、肾近曲小管、大脑尾状核、精神分裂症脑S-11额叶、全脑、丘脑中含量丰富。结论HIV—ep2／MIBP1／MBP2蛋白为一个2500氨基酸的巨大蛋白，主要结构为4个锌指，定位于细胞核，可能与cyclin系统、Src家族等有关。

The bioinfomatics study of the structure and function of HIV-ep2/MIBP1/MBP2 protein

Objective To study the structure and function of HIV-ep2/MIBP1/MBP2 protein. Methods Searched the GeneBank database for the whole sequence of HIV-ep2/MIBP1/MBP2 protein. Computed the basic character of it by ProtParam and the 2~(nd) structure on the ExPASy web site.Predicted the probable domains with SMART tool,set up 3~(rd)structure with the PDB tool,predicted the motifs with ELM. Analyzed the subcellular locus and the phosphalyzation sites,searched for the tissue expression character on SOURSE web site.Results A gigantic protein composed of 2500 aa is expressed by HIV-ep2/MIBP1/ MBP2 gene which is an unstable protein containing 62.52% ransom coils;22.36%a helix;10.32% extend strand ;4.80% 13 turn.In the 3~(rd) structure,there were 4 C2 H2 Zinc finger tound whose 3-dimentional feature were established.26 candid motifs were predicted.This protein were localized in cell nucleolus,expressed significantly in follicular lymphoma,the approximate renal tubules,the caudal nucleus,the frontal lobe of schizophrenia S-11 brain,total brain,thalamus.Conclusion The HIV-ep2/MIBP1/MBP2 protein is a gigantic protein containing 2500 aa,the main domains is 4 C_2H_2 Zinc finger structure,localized in cell nucleolus,abundant in brain,have potent close relation with cyclin system and Src family.