辽宁省艾滋病病毒1流行株膜蛋白V3环氨基酸变异特点

目的　了解辽宁省艾滋病病毒 1(HIV 1)感染者 艾滋病 (AIDS)患者体内不同亚型HIV 1膜蛋白V3环氨基酸序列特征 ,突变种类特点。方法　提取含有整合HIV 1前病毒的基因组DNA ,巢式聚合酶链反应 (nest PCR)扩增后直接测序 ,并做序列排列比对、翻译和分析。结果　辽宁省HIV 1感染者感染病毒分属A、B’、C、G四种亚型 ,AIDS患者体内病毒V3环发生与T嗜性 SI表型有关的氨基酸突变形式 (11位出现R ,13位出现S、T、N ,19位出现V ,2 0位出现Y ,2 5、2 9位出现N等 )高于无症状感染组 (P <0 .0 5 ) ,此外还发现GQGR、APGQ、RPGA、GLGR、RPGA等少见的V3环顶端四肽组成形式及第 5位H ,34位S、F等罕见突变形式。结论　辽宁省AIDS人群中 ,A、B、C、G亚型HIV 1毒株V3环各位置出现氨基酸突变情况与国外对B亚型毒株的研究结果总体上相似 ,但发现一些罕见突变和少见V3环顶端四肽组成形式。

Mutation of envelop protein V3 loop in HIV- 1 epidemic in Liaoning province

Objective To study the characteristics of human immunodeficiency virus (HIV-1) V3 loop amino acid mutations among HIV-1 infected people in Liaoning province. Methods The whole blood samples of the HIV carriers and AIDS patients were collected in Liaoning province, China and were extracted PBMC genome DNA. HIV-1 V3 and flanking region sequences were amplified by nest-polymerase chain reaction (nest-PCR) with env specific primers: ED5/ED12 and ED31/ED33. Products were sequenced directly and sequences were aligned, translated and analyzed. Results In AIDS group, some amino acid mutations at specific position of V3 loop: S to R at position 11, H to S,T and N at position 13, A to V at position 19, F to Y at position 20, Q or D to N at position 25 and 29, were found and all common mutations were associated with T tropic/SI phenotype. The frequency of such amino acid mutations in specific positions was higher in AIDS group than that of the asymptomatic infection group ( P < 0.05 ). In addition, we found some unusual tetramer compositions on the tip of V3 loop: GQGR, APGR and RPGA, GLGR, RPGA in addition to some rare mutations, such as: N to H at position 5 and H to S, F at position 34. Conclusion The amino acid mutations on the V3 loop of HIV-1 epidemic in Liaoning province were in agreement with the results of subtype B, but we observed some rare mutations and unusual tetramer compositions on the tip of V3 loop.