不同梅毒螺旋体基因重组抗原酶联免疫试验比较

目的 对单一梅毒螺旋体(Treponema pallidum,Tp)基因重组抗原Tp47、Tp42或Tp17及联合应用3种基因重组抗原(Tp47、Tp42和Tp17)酶联免疫试验(enzyme immunoassay,EIA)进行比较.方法 分别用3种单一Tp基因重组抗原Tp47、Tp42或Tp17EIA法,检测11份梅毒血凝试验Tp抗体阳性和28份阴性血清,并与联合应用3种基因重组抗原(Tp47、Tp42和Tp17)EIA法比较.结果 单一基因重组抗原Tp47、Tp42或Tp17EIA法的灵敏度分别为100%(11/11)、100%(11/11)和90.9%(10/11),特异度分别为964%(27/28)、100%(28/28)和964%(27/28);联合应用3种基因重组抗原EIA法的灵敏度和特异度分别为100%(11/11)和100%(28/28).结论 单一Tp基因重组抗原EIA法用于梅毒诊断存在一定的假阳性和假阴性反应,联合应用3种基因重组抗原(Tp47、Tp42和Tp17)EIA法的灵敏度高于单一Tp基因重组抗原EIA法.

Comparison of Different Recombinant Antigen-Based Enzyme Immunoassays for Detection of Antibodies Against Treponema Pallidum

Objective To compare the enzyme immunoassays(EIA)based on a single recombinant T reponema pallidum (TP)antigen Tp47,Tp42 or Tp17 with that using combination of the three recombinant Tp ant igens.Methods Anti-Tp negaove and 10 anti-Tp positive sera tested by TPHA(Treponema pallidum hemagg lutination)were detected by both the EIA based on combination of the 3 recombinant Tp antigens(Tp47,Tp42 and Tp17)and the EIA using a single Tp protein (Tp47,or Tp42,or Tp17).Results The sensitivity of EIA based on a single antigen Tp47,Tp42,or Tp17 was 100%(11/11),100%(11/11)and 90.9%(10/11),with the speciacity of 96.4%(27/28),100%(28/28)and 96.4%(27/28),respectively.The sensitivity and the specificity of EIA based on combination of the three Tp antigens were 100%(11/11)and 100%(28/28),respectively.Conclusion There was some false negative and false positive reactivity with the EIA based on a single recombinant antigen for diagnosis of syphilis.The sensitivity of EIA based on combination of the three antigens (Tp47,Tp42 and Tp17)for detection of anti-Tp antibody was higher than that of the EIA with a single recombinant Tp antigen.