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Functional and antigenic properties of cultured T cells in the cell mediated lympholysis (CML) assay |
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| Authors: | Karen S. Zier |
| Affiliation: | From the Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, USA |
| Abstract: | Cultured T cells (CTC) were expanded in Interleukin-2 (IL-2) and used as reagents in cell mediated lympholysis (CML). CTC were able to induce the generation of primary cytotoxic effector cells and to function as 51Cr labeled target cells and cold target inhibitors. Since large numbers of CTC can be produced from as few as 1–2 × 106 lymphocytes within a short period of time, these reagents with enable CML studies to be performed in a broader range of situations including those which were heretofore impossible. |
| Keywords: | IL-2 Interleukin-2 HLA human leukocyte antigens CTC cultured T cells MLC mixed leukocyte culture PBL peripheral blood lymphocytes PHA phytohemagglutinin CML cell mediated lympholysis tritiated thymidine CTL cytotoxic T lymphocytes |
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