Raman spectroscopy for optical diagnosis in the larynx: preliminary findings |
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Authors: | Lau David P Huang Zhiwei Lui Harvey Anderson Donald W Berean Ken Morrison Murray D Shen Liang Zeng Haishan |
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Institution: | Department of Otolaryngology, Singapore General Hospital, Singapore 169608, Singapore. gollpc@sgh.com.sg |
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Abstract: | BACKGROUND AND OBJECTIVES: Raman spectroscopy (RS) provides information about molecular structure and is a potential tool for non-invasive tissue diagnosis. To determine if Raman spectra could be obtained rapidly from laryngeal tissue in vitro, and compare Raman spectra from normal, benign, and cancerous laryngeal tissue. STUDY DESIGN/MATERIALS AND METHODS: Forty-seven laryngeal specimens were studied using RS with signal acquisition times (SAT) between 1 and 30 second(s). Multivariate analysis was used to determine the diagnostic ability of RS compared to standard histology (n = 18, 13, and 16 respectively for normal tissue, carcinoma, and squamous papilloma). RESULTS: Good quality spectra were obtained with 5-second SAT. Spectral peak analysis showed prediction sensitivities of 89%, 69%, and 88%, and specificities of 86%, 94%, and 94% for normal tissue, carcinoma, and papilloma. CONCLUSIONS: In the larynx, spectral differences appear to exist between normal tissue, carcinoma, and papilloma. The ability to obtain spectra rapidly supports potential for future in vivo studies. |
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Keywords: | Raman spectroscopy larynx non‐invasive diagnosis |
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