| 双酶法合成D—（—）—对羟基苯甘氨酸 |
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| 引用本文： | 双酶法合成D—（—）—对羟基苯甘氨酸[J]. 中国药科大学学报, 2001, (2): 75-78. |
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| 作者姓名： | 马飞 许激扬 何林松 李隽 |
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| 作者单位： | 中国药科大学生物化学教研室,南京,210009 |
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| 摘 要： | 目的 研究用土壤杆菌CPU1295完整细胞双酶转化法生产D－（－）－对羟基苯甘氨酸。方法 通过测定D－因酶及D－氨甲酰酶的比活力来选择发酵和转化反应条件。结果 经优化，两酶化活力分别达到1.3u/ml发酵液和0.46u/ml发酵液，终产物经鉴定为D－（－）－对羟基苯甘氨酸。结论 本法是一种有发展前途的生产工艺。
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| 关 键 词： | D－对羟基苯甘氨酸 对羟基苯乙内酰脲 D－海因酶 D－氨甲酰酶 双酶法 合成 |
| 文章编号： | 1000-5048(2001)02-0155-21 |
| 修稿时间： | 2000-09-14 |
Dual-enzymatic Synthesis of D-(-)-p-Hydrox yphenylglycine |
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| Dual-enzymatic Synthesis of D-(-)-p-Hydrox yphenylglycine[J]. Journal of China Pharmaceutical University, 2001, (2): 75-78. |
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| Authors: | MA Fei XU Ji-Yang HE Lin-Song LI Jun |
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| Affiliation: | MA Fei,XU Ji-Yang,HE Lin-Song,LI Jun Department of Biochemistry,China Pharmaceutical University,Nanjing 210009 |
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| Abstract: | AIM To investigate the process of producing D-p-hydroxyphenylglycine from DL-p-hydroxy-phenylhydantoin via the catalysis of intact cells of Agrobacterium sp. CPU 1295. METHODS The fermentation and conversion conditons were chosen by means of determining the D-hydantoinase and N-carbamoylase specific activity. RESULTS Under the optimized conditions 1.3 u/ml of D-hydantoinase specific activity and 0.46 u/ml of N-carbamoylase specific activity were achieved. CONCLUSION This process is feasible for the manufacture of D-p-hydroxyphenylglycine. |
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| Keywords: | D-p-hydroxyphenylglycine p-hydroxyphenylhydantion Agrobacterium D-hydantoinase N-carbamylase |
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