复黄生肌愈创油膏对大鼠慢性皮肤溃疡模型bFGF EGFmRNA表达的影响

目的:探讨外用中药-复黄生肌愈创油膏(复黄膏)促进慢性皮肤溃疡创面修复的机制.方法:用醋酸氢化可的松造成SD大鼠背部慢性体表溃疡模型,随机分为模型对照组、复黄膏治疗组(复1组)、小剂量复黄膏治疗对照组(复2组)、生肌白玉膏(白玉膏)治疗对照组(白玉组)和模型组.采用RT-PCR方法检测各组创面组织中EGFmRNA和bFGFmRNA表达水平;流式细胞仪测定创面组织细胞DNA周期.结果:复黄1组创面组织中EGFmRNA和bFGFmRNA表达水平与模型组、白玉组、复2组相比呈明显上调,P<0.05,复1组S期细胞数与模型组相比差异有显著意义(P<0.05).结论:复黄膏促进慢性皮肤溃疡愈合作用与上调EGFmRNA和bFGFmRNA表达水平有关.

Influence of Fu-Huang-Sheng-Ji-Yu-Chuang Ointment on the Expression of EGFmRNA and bFGFmRNA in the Rat Model of Chronic Skin Ulcer

Objective:To explore the mechanism of Fu-Huang-Sheng-Ji-Yu-Chuang Ointment (or Fu-Huang Ointment),an external TCM remedy,healing chronic skin ulcer.Method:By injection of hydrocortisone acetate,chronic skin ulcer was made in the back of SD rats.The rats were divided randomly into comparison group,Fu-Huang-Sheng-Ji-Yu-Chuang Ointment group with routine dosage(Fu-Huang 1 group),Fu-Huang-Sheng-Ji-Yu-Chuang Ointment group with small dosage(Fu-Huang 2 group),Sheng Ji Bai Yu Ointment group(Bai Yu Ointment group)and model group.RT-PCR method is used to measure the quantity of EGFmRNA and bFGFmRNA.And the DNA period of ulcer tissue cell is measurated by Flow Cytometry detector.Results:In Fu-Huang 1 group,the expressions of EGFmRNA and bFGFmRNA in the ulcer tissue were increased significantly than those of model group,Bai Yu Ointment group and Fu-Huang 2 group(P<0.05),and the cell of Speriol were improved significantly than that of model group(P<0.05),Conclusion:The speeding effect of Fu-Huang Ointment in healing chronic skin ulcer is related to the expressions of EGFmRNA and bFGFmRNA.