应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究

目的： 利用液相色谱-飞行时间质谱 (liquid chromatography-time of flight- mass spectrography，LC/TOF-MS)检测食管鳞状细胞癌 (esophageal squamous cell carcinoma，ESCC)血浆中小分子代谢物质的变化，探讨与ESCC相关的小分子代谢标志物。方法：收集ESCC和健康对照血浆样本各30例，应用LC/TOF-MS方法检测，采用MassHunter软件对数据进行分子特征提取，MPP软件对其进行分析，对可能的代谢物精确质量在METLIN公共数据库中进行搜索查询。结果：与健康对照组相比，ESCC组共找到差异表达的血浆小分子代谢物数目46个，其中13个上调，33个下调。经过METLIN数据库搜索后，ESCC血浆中的磷脂酸、磷脂酰丝氨酸、磷脂酰甘油碎片含量与健康对照组相比显著降低。结论：基于LC/TOF-MS的血浆小分子代谢物分析策略可有效区分食管癌和健康个体，在食管癌的早期诊断和人群筛检方面具有较好的应用前景。

A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS

OBJECTIVE： The plasma low-molecular weight metabolites of esophageal squamous cell carcinoma were analyzed by liquid chromatography-time of flight-mass spectrography（ LC/TOF-MS） to explore potential biomarkers for the early detection of ESCC. METHODS：Plasma samples were obtained from 30 ESCC patients and 30 healthy volunteers,low-molecular weight metabolites were identified by LC/TOF-MS,feature detection was performed by MassHunter software MFE algorithm,statistical analysis was conducted by MPP software,and primary identification was achieved by searching METLIN database. RESULTS：Compared to healthy controls,46 differentially expressed features were detected,13 of which were up-regulated in ESCC group,while the other 33 were down-regulated. After searching with METLIN database,plasma levels of PA,PS,PG were significantly lower in ESCC group. CONCLUSION：ESCC patients are markedly different from healthy people in plasma metabolite composition,and the LC/TOF-MS plasma metabonomics shed light on esophageal cancer early detection and screening.