Monoclonal antibodies monospecific to single-chain urokinase-type plasminogen activator (scu-PA) |
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| Authors: | J G Scheuerlein I Kalies W P Gluth J R Kalden |
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| Affiliation: | Institut für Klinische Immunologie und Rheumatologie der Universitat Erlangen-Nürnberg, FRG. |
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| Abstract: | Until now, single-chain urokinase-type plasminogen activator (scu-PA) could not be distinguished from urokinase (UK) by immunological methods. Therefore, in our study monoclonal antibodies (mAbs) specific for scu-PA were raised in mice. These mAbs proved to discriminate between scu-PA, UK and plasmin-activated scu-PA. The clones producing these mAbs could be cultivated in a serum-free medium. Furthermore, very pure preparations were obtained by a one-step purification procedure on protein-A-sepharose. The mAbs described provide a future potential for immuno-affinity chromatography and for the specific determination of scu-PA. |
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