绝经前、后EP中ER、PR、Bcl-2、Ki-67蛋白表达特点

目的:探讨绝经前、绝经后子宫内膜息肉(EP)中雌激素受体(ER)、孕激素受体(PR)、B-细胞淋巴瘤基因蛋白(Bcl-2)、细胞核相关抗原(Ki-67)的表达,为研究EP形成机制提供思路。方法:随机选取绝经前、绝经后EP标本各15例,及绝经前正常增生期子宫内膜和绝经后正常萎缩型子宫内膜各10例,共50例标本。用生物素蛋白免疫组化法(SP法)检测各组织上EP、PR、Bcl-2、Ki-67的表达情况,并进行统计分析。结果:①绝经前组:PR在EP腺体和间质的表达均低于其在正常增生期子宫内膜中的表达(P<0.05),Bcl-2在EP腺体和间质的表达均高于其在正常增生期子宫内膜中的表达(P<0.05),ER、Ki-67在EP和正常增殖期子宫内膜中的表达无差异(P>0.05);②绝经后组:ER、PR、Bcl-2在EP间质和腺体中的表达均高于三者在绝经后正常萎缩型子宫内膜中的表达(P<0.05),Ki-67在EP和绝经后正常萎缩型内膜中的表达没有差异;③PR在绝经后EP腺体及间质的表达显著高于其在绝经前EP中的表达(P<0.05),ER、Bcl-2、Ki-67在绝经前、后EP中的表达没有显著性差异(P>0.05)。结论:子宫内膜局部ER、PR的分布不均和功能改变在EP的形成中可能起到一定作用;局部细胞凋亡的异常在EP的形成中可能起到一定作用。

Experimental study of the expression of ER,PR,Bcl-2 and Ki-67 in EP from pre-and post-menopausal women

Objective: To detect the expression of ER,PR,Bcl-2 and Ki-67 in EP from pre-and post-menopausal women and investigate the pathogenesis of EP.Methods: Collected 30 cases of EP(15 from pre-menopausal women and the other 15 from post-menopausal women)and 20 cases of normal endometrium(10 were proliferative phase endometrium and the other 10 were atrophic endometrium).Examined the expression of ER、PR、Bcl-2 and Ki-67 in each tissue by Streptavidin peroxidase immunohistochemistry.Then analyzed the data with statistics method.Results: ①In the pre-menopausal group: the expression of PR in EP(both in gland and stromal cells) was weaker than that in proliferative phase endometrium(P<0.05),the expression of Bcl-2 in EP(both in gland and stromal cells) was higher than that in proliferative phase endometrium(P0.05),and the expression of ER and Ki-67 between in EP and in proliferative phase endometrium,had no significant differences(P>0.05);②In the post-menopausal group: the expressions of ER,PR and Bcl-2 in EP(both in gland and stromal cells) were higher than that in atrophic endometrium(P<0.05),and the expression of Ki-67 between in EP and in atrophic endometrium,had no significant difference(P>0.05);③The expression of PR in EP from post-menopausal was higher than that in EP from pre-menopausal(P<0.05),there were no significant differences on the expression of ER,Bcl-2 and Ki-67 in EP between pre-and post-menopausal women(P>0.05).Conclusion The abnormal of distribution and function of ER and PR in local endometrium may have some effect on the genesis of EP;the abnormal of cells apoptosis in local endometrium may have some effect on the genesis of EP.