参蒌双心丸对大鼠心肌缺血再灌注损伤的保护作用

目的:观察参蒌双心丸对大鼠心肌缺血再灌注损伤的保护作用。方法:将80只雄性Wistar大鼠,随机分为假手术组、模型组、合心爽组、丹蒌片组及参蒌双心丸0.35,0.7,1.4,2.8 g·kg-1组,各组大鼠采用冠状动脉左前降支穿线后即刻经给药,稳定10 min后结扎冠状动脉左前降支45 min,再灌注180 min的方法进行造模,假手术组只穿线不结扎,造模后,观察心肌梗死范围,检测血清肌酸激酶(CK),肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)的变化。结果:与假手术组比较,模型组大鼠心肌梗死范围及血清中CK,CK-MB和LDH水平显著升高(P0.01);与模型组比较,除参蒌双心丸0.7 g·kg-1组,其余各组心肌梗死范围差异均有统计学意义(P0.05,P0.01);与模型组比较,除参蒌双心丸2.8 g·kg-1组外,其余各组大鼠血清CK,LDH,CK-MB水平明显下降(P0.05,P0.01)。结论:参蒌双心丸可通过减小心肌梗死面积,降低血清心肌酶水平,发挥对大鼠心肌缺血再灌注损伤的保护作用。

Protective Effect of Shenlou Shuangxin Pills on Myocardial Ischemia Reperfusion Injury in Rats

Objective: To observe the protective effects of Shenlou Shuangxin(SLSX) pills on the myocardial ischemia reperfusion injury in rats. Method: Totally 80 male Wistar rats were randomly divided into sham operation group, model group, diltiazem group, danlou tablets group, and SLSX pills (0.35,0.7,1.4,2.8 g·kg^-1) groups. The model was induced by threading the left anterior descending coronary artery in each group, instantly giving the drugs, 10 min later ligating the left anterior for 45 min, and reperfusing for 180 min. Sham operation group was only threaded, but not ligated. After the modeling, efforts were made to observe the changes in myocardial infarct range, serum creatine kinase (CK), creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH). Result: Compared with sham operation group, model group showed significant increases in CK, CK-MB, LDH and myocardial infarct range (P<0.01); Compared with the model group, all SLSX pills groups had significant differences in myocardial infarct range, except for SLSX pills 0.7 g·kg^-1 group (P< 0.05,P<0.01); Compared with the model group, all SLSX pills groups showed obvious declines in serum CK, LDH and CK-MB, except for SLSX pills 2.8 g·kg^-1 group (P<0.05,P<0.01). Conclusion: SLSX pills has the protective effect on myocardial ischemia reperfusion injury in rats by reducing the myocardial infarct area and the level of serum myocardial enzyme.