CD40基因启动子区-1C／T多态性与早发急性心肌梗死的相关性研究

目的：研究我国湖北十堰地区汉族人群CD40基因启动子区-1C／T多态性与早发急性心肌梗死（AMI）的相关性．方法：应用流式细胞仪俭测161例早发AMI患者和186名健康对照者的B淋巴细胞CD40表达水平；用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测CD40基因启动子区-1C／T多态性的基因型频率分布；应用酶联免疫吸附试验检测血清可溶性CD40配体（sCD40L）水平；应用微粒子增强透射免疫分析技术检测血清超敏C-反应蛋白（hs-CRP）水平？结果：早发AMI患者B淋巴细胞CD40表达水平高于对照组，且差异有统计学意义（平均荧光强度为6．81±2．16比2．08±1．19．P〈0．01）：而其血清sCD40L及hs-CRP也均较对照组升高，差异有统计学意义（P均〈0．01）。基因型分布在早发AMI组（CC=34.2％、CT=54．7％、TT=11．1％）与对照组（CC=23．7％、CT=56．9％、TT：19．4％）的差异有统计学意义（P〈0．05）：早发AMI组C等位基因频率高于对照组，差异有统计学意义（61．5％比52．2％，OR=L465，95％CI：1．082-1．983，P〈0．05）；B淋巴细胞CD40表达水平在CC、CT、TT各基因型间差异也有统计学意义（P〈0．05）。结论：CD40基因启动子区-1C／T多态性可能与我国湖北十堰地区汉族人群早发AMI的发生有关。

Relationship between CD40 gene promoter region-1C/T polymorphism and early onset acute myocardial infarction

Objective To investigate the relationship between CD40 gene promoter region -1C/T polymorphism and early onset acute myocardial infarction （AMI） in Hans from Shiyan, Hubei Province of China. Methods One hundred and sixty-one definite early onset AMI patients （AMI group） and 186 healthy controls （control group） were recruited. The expression level of CD40 in B lymphoeytes was determined by flow cytometry and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to determine the genotype frequency distribution of CD40-1C/T polymorphism. Serum soluble CD40 ligand （sCD40L） was measured by ELISA and serum hypersensitive C-reactive protein （hs-CRP） was determined by particle enhanced-turbidimetric immunoassay. Results Expression level of CD40 was higher in the AMI group than that in the control group （mean fluorescence intensity 6.81±2.16 vs 2.08±1.19, P〈0.01）; serum sCD40L and hs-CRP were obviously elevated in the AMI group. The difference of genotype distribution was statistically significant between AMI group （CC=34.2%, CT=54.7%, 3T=l 1.1%） and the control group （CC=23.7%, CT=56.9%, TF= 19.4%）. The fiequency of C allele was higher in the AMI group than that in the control group （61.5% vs 52.2%, OR= 1.465,95%CI：1.082-1.983,P〈0.05）. Conclusions CD40 gene promoter region-1C/T polymorphism might be correlated with the development of early onset AMI in Hans from Shiyan, Hubei Province of China.