| p^53基因与Rb基因在卵巢癌组织中突变的初步分析 |
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| 引用本文： | 蔡公丽 王芳. p^53基因与Rb基因在卵巢癌组织中突变的初步分析[J]. 中国优生与遗传杂志, 1998, 6(3): 15-17,20 |
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| 作者姓名： | 蔡公丽 王芳 |
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| 作者单位： | Dalian 210 Hospital，116021 |
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| 摘 要： | 为了解卵巢癌组织中抗癌基因Ｐ＾５３与Ｒｂ基因的突变情况，我们把ＰＣＲ单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术及ＰＣＲ－ＤＮＡ直接测序方法应用于５７例卵巢癌组织细胞的检测，结果发现ｐ＾５３基因可能的突变率为３２％，Ｒｂ基因可能的突变率为２１％，不同分化程度肿瘤的Ｐ５３及Ｒｂ基因突变率未见明显区别，结论：ＰＣＲ－ＳＳＣＰ角染技术是筛查基因突变简便，敏感，无核素污染的最佳方法，而ＰＣＲ－ＤＮＡ直接测序仪
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| 关 键 词： | 卵巢癌 P53基因 Rb基因 PCR－SSCP |
Preliminary analysis of p53 gene and Rb gene mutation in ovarian cancer tissue. |
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| Cai Gongli,Yu Li,Pu Lili.. Preliminary analysis of p53 gene and Rb gene mutation in ovarian cancer tissue.[J]. Chinese Journal of Birth Health & Heredity, 1998, 6(3): 15-17,20 |
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| Authors: | Cai Gongli Yu Li Pu Lili. |
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| Abstract: | Objective: To recognize the mutation status of p53 and Rb cancer suppresor genes. Methods: for the first time, 57 ovarian cancer samples were analysised by PCR for DNA amplification, and then SSCP, furthes PCR DNA sequence. Results:(1) The mutation ratio of p53 in ovarian cancer was 32%, (2) The mutation of Rb gene in ovarian cancer was 21%, (3)Neither p53 nor Rb gene mutation ratio was found significant difference in varions differential stages. Conclusions: PCR SSCP DNA sequence method is the best one in testing gene mutation for its simplicity, sensitivity and no radioisotope contamination. Direct PCR DNA sequence can test point mutation of single amino acid change. |
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| Keywords: | Ovarian cancer p53 gene Rb gene PCR SSCP Direct PCR DNA sequence. |
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