蒙脱石散和美沙拉秦对溃疡性结肠炎大鼠血中VIP、SP、5-HT的影响

目的探讨美沙拉秦和蒙脱石散（思密达）对溃疡性结肠炎（UC）大鼠血中血管活性肠肽（VIP）、P物质（SP）、五羟色胺（5-HT）的影响.方法 82只SD雄性大鼠,随机分为正常组、模型组、干预组,干预组包括思密达组、美沙拉秦组,美沙拉秦加思密达组.采用2,4,6三硝基苯磺酸（TNBS）/乙醇法建立大鼠溃疡性结肠炎模型.确认模型建立后,模型组每天经口灌入生理盐水,干预组分别每天给予思密达、美沙拉秦、美沙拉秦联合思密达治疗.每天对模型组、干预组大鼠进行疾病活动指数（DAI）评分.于第5天、第12天每组随机抽取6只大鼠收集血标本.采用放射免疫分析法测定血中VIP、SP,采用ELISA法测血中5-HT的含量.结果DAI评分结果示,5 d的炎症评分并无差异.12 d的中模型组与思密达组炎症重于美沙拉秦组和美沙拉秦加思密达组（P〈0.05）.5 d组中,模型组VIP低于正常组（P〈0.01）,模型组与干预组的SP高于正常组（P〈0.01）.12 d的模型组SP、5-HT高于正常组（P〈0.05）;仅美沙拉秦组的SP与正常组无差异（P〉0.05）,其他干预组均高于正常（P〈0.01）.12 d模型组、思密达组的5-HT均高于美沙拉秦组、美沙拉秦加思密达组（P〈0.05）.结论 UC经治疗后,单用美沙拉秦、美沙拉秦联合思密达用药抗炎近期效果较单用思密达近期效果好,5 d血中SP浓度值升高能及时反应炎症情况,12 d血中5-HT变化能反应治疗后炎症的变化.5 d血中SP和12 d血中5-HT的测定可能为急性期UC的病情发展和临床治疗急性期UC判断疗效提供重要的参考依据.

Effect of Mesalazine and Smecta on Plasma VIP,SP and 5-HT in Rats with Ulcerative Colitis

Objective To explore the effect of Mesalazine or/and Smecta on the plasma vasoactive intestinal peptide（VIP）,Substance P（SP） and serum 5-hydroxytryptamine（5-HT） in the rats with ulcerative colitis（UC）. Methods 82 male SD rats were randomly divided into normal group, model group and intervention group including Smecta group, Mesalazine group, Mesalazine and Smecta group.The rat models with UC were established by 2,4,6 three trinitrobenzene sulfonic acid（TNBS） /ethanol method. The model group was daily treated with the saline,and the intervention group was daily treated with Smecta,Mesalazine,Mesalazine and smecta. The model group and intervention group rats were given diseases activity index（DAI） score every day. 6rats were randomly selected in each group on 5th day and 12 th day. The pericardial punctures were operated and blood specimens were collected after anesthetized. The content of VIP and SP in the plasma was measured by radioimmunoassay method, and The content of 5-HT in the serum was measured by ELISA method. Results DAI score results showed the inflammation score on the 5th day had no difference among groups. The inflammation on the 12th day in model group and Smecta group were serious than in Mesalazine group and Mesalazine with Smecta group（P〈0.05）. On the 5th day,the plasma VIP concentration in the model group was lower than the normal group（P〈0.05）,The plasma SP concentration was higher in the model group and intervention group than in the normal group（P〈0.05）. On the 12 th day, The plasma VIP, SP and the serum 5-HT concentrations were higher in the model group than in the normal group（P〈0.05）,which were as same as the intervention groups except Mesalazine group. the serum 5-HT concentration was higher on the 12 th day in the model group and Smecta group than in Mesalazine group and Mesalazine with Smecta group（P〈0.05）.Conclusions In the rats with ulceractive colitis and after the intervention, single only oral Mesalazine and combination of the M