High rates of excitatory miniature currents in crayfish claw opener muscle evoked by high concentrations of gamma-aminobutyric acid (GABA) in normal and Ca2+-deficient superfusions |
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Authors: | W Finger |
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Institution: | Physiologisches Institut der Technischen Universität München, Biedersteiner Str. 29, D-8000 München 40 F.R.G. |
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Abstract: | High concentrations (0.5 mol/l) of the neutral amino acid GABA were used to evoke release of transmitter quanta from excitatory terminals at voltage clamped crayfish muscle fibres in normal and Ca2+-deficient superfusions. An experiment in which the release of transmitter quanta proceeded at high rates in both normal and Ca2+-deficient superfusion was analyzed in detail indicating a Ca2+-independent mechanism of release. In the normal superfusion, on application of GABA, the release rates ñ increased within a few seconds up to about 6000 quanta/s and thereafter declined exponentially with a time constant , most likely due to depletion of a readily releasable store of transmitter in the excitatory nerve terminals comprising at least 110,000 quanta per muscle fibre. Assuming that about 1900 excitatory synapses exist per muscle fibre 9], it results that about 58 quanta can be associated with each synapse in agreement with morphological data 15] which show that between 47–117 vesicles exist in a single glutamatergic synapse of crayfish. |
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Keywords: | crayfish claw opener muscle GABA-evoked transmitter release excitatory miniature currents calcium dependence of release presynaptic store of transmitter depletion noise analysis |
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